Stem Cell Research (Jul 2020)
Generation of twenty four induced pluripotent stem cell lines from twenty four members of the Lothian Birth Cohort 1936
- Jamie Toombs,
- Lindsay Panther,
- Loren Ornelas,
- Chunyan Liu,
- Emilda Gomez,
- Raquel Martín-Ibáñez,
- Simon R. Cox,
- Stuart J. Ritchie,
- Sarah E. Harris,
- Adele Taylor,
- Paul Redmond,
- Tom C. Russ,
- Lee Murphy,
- James D. Cooper,
- Karen Burr,
- Bhuvaneish T. Selvaraj,
- Cathy Browne,
- Clive N. Svendsen,
- Sally A. Cowley,
- Ian J. Deary,
- Siddharthan Chandran,
- Tara L. Spires-Jones,
- Dhruv Sareen
Affiliations
- Jamie Toombs
- Centre for Discovery Brain Sciences, UK Dementia Research Institute, The University of Edinburgh, UK
- Lindsay Panther
- iPSC Core, The David Janet Polak Foundation Stem Cell Core Laboratory, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA; Cedars-Sinai Biomanufacturing Center, West Hollywood, CA 90069, USA
- Loren Ornelas
- iPSC Core, The David Janet Polak Foundation Stem Cell Core Laboratory, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA; Cedars-Sinai Biomanufacturing Center, West Hollywood, CA 90069, USA
- Chunyan Liu
- iPSC Core, The David Janet Polak Foundation Stem Cell Core Laboratory, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA; Cedars-Sinai Biomanufacturing Center, West Hollywood, CA 90069, USA
- Emilda Gomez
- iPSC Core, The David Janet Polak Foundation Stem Cell Core Laboratory, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA; Cedars-Sinai Biomanufacturing Center, West Hollywood, CA 90069, USA
- Raquel Martín-Ibáñez
- Cedars-Sinai Biomanufacturing Center, West Hollywood, CA 90069, USA
- Simon R. Cox
- Lothian Birth Cohorts, Department of Psychology, University of Edinburgh, Edinburgh, UK
- Stuart J. Ritchie
- Lothian Birth Cohorts, Department of Psychology, University of Edinburgh, Edinburgh, UK
- Sarah E. Harris
- Lothian Birth Cohorts, Department of Psychology, University of Edinburgh, Edinburgh, UK
- Adele Taylor
- Lothian Birth Cohorts, Department of Psychology, University of Edinburgh, Edinburgh, UK
- Paul Redmond
- Lothian Birth Cohorts, Department of Psychology, University of Edinburgh, Edinburgh, UK
- Tom C. Russ
- Lothian Birth Cohorts, Department of Psychology, University of Edinburgh, Edinburgh, UK; Alzheimer Scotland Dementia Research Centre, University of Edinburgh, Edinburgh, UK
- Lee Murphy
- Edinburgh Clinical Research Facility, University of Edinburgh, Edinburgh, UK
- James D. Cooper
- Dementia Research Institute at the University of Edinburgh, UK; Centre for Clinical Brain Sciences, University of Edinburgh, Edinburgh, Scotland, UK
- Karen Burr
- Dementia Research Institute at the University of Edinburgh, UK; Centre for Clinical Brain Sciences, University of Edinburgh, Edinburgh, Scotland, UK
- Bhuvaneish T. Selvaraj
- Dementia Research Institute at the University of Edinburgh, UK; Centre for Clinical Brain Sciences, University of Edinburgh, Edinburgh, Scotland, UK
- Cathy Browne
- James Martin Stem Cell Facility, Sir William Dunn School of Pathology, University of Oxford, Oxford, UK
- Clive N. Svendsen
- Board of Governors-Regenerative Medicine Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA; Department of Biomedical Sciences, Cedars-Sinai Medical Center, Los Angeles, CA, 90048, USA
- Sally A. Cowley
- James Martin Stem Cell Facility, Sir William Dunn School of Pathology, University of Oxford, Oxford, UK; Oxford Parkinson’s Disease Centre, Oxford, UK
- Ian J. Deary
- Lothian Birth Cohorts, Department of Psychology, University of Edinburgh, Edinburgh, UK
- Siddharthan Chandran
- Dementia Research Institute at the University of Edinburgh, UK; Centre for Clinical Brain Sciences, University of Edinburgh, Edinburgh, Scotland, UK
- Tara L. Spires-Jones
- Centre for Discovery Brain Sciences, UK Dementia Research Institute, The University of Edinburgh, UK; Corresponding authors at: iPSC Core, Cedars-Sinai Biomanufacturing Center, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA (D. Sareen); Centre for Discovery Brain Sciences, UK Dementia Research Institute, The University of Edinburgh, Edinburgh EH8 9JZ, UK (T.L. Spires-Jones).
- Dhruv Sareen
- iPSC Core, The David Janet Polak Foundation Stem Cell Core Laboratory, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA; Cedars-Sinai Biomanufacturing Center, West Hollywood, CA 90069, USA; Board of Governors-Regenerative Medicine Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA; Department of Biomedical Sciences, Cedars-Sinai Medical Center, Los Angeles, CA, 90048, USA; Corresponding authors at: iPSC Core, Cedars-Sinai Biomanufacturing Center, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA (D. Sareen); Centre for Discovery Brain Sciences, UK Dementia Research Institute, The University of Edinburgh, Edinburgh EH8 9JZ, UK (T.L. Spires-Jones).
- Journal volume & issue
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Vol. 46
p. 101851
Abstract
Cognitive decline is among the most feared aspects of ageing. We have generated induced pluripotent stem cells (iPSCs) from 24 people from the Lothian Birth Cohort 1936, whose cognitive ability was tested in childhood and in older age. Peripheral blood mononuclear cells (PBMCs) were reprogrammed using non-integrating oriP/EBNA1 backbone plasmids expressing six iPSC reprogramming factors (OCT3/4 (POU5F1), SOX2, KLF4, L-Myc, shp53, Lin28, SV40LT). All lines demonstrated STR matched karyotype and pluripotency was validated by multiple methods. These iPSC lines are a valuable resource to study molecular mechanisms underlying individual differences in cognitive ageing and resilience to age-related neurodegenerative diseases.
