Folia Histochemica et Cytobiologica (Jan 2009)

Co-transfection of EYFP-GH and ECFP-rab3B in an experimental pituitary GH3 cell: a role of rab3B in secretion of GH through porosome.

  • Hiroshi Nakaguchi,
  • Junichi Tanaka,
  • Shuichiro Asano,
  • Takeshi Uno,
  • Satoru Miyawaki,
  • Fuyuaki Ide,
  • Hiroko Okinaga,
  • R Yoshiyuki Osamura,
  • Susumu Takekoshi,
  • Akiko Mizutani,
  • Johbu Itoh,
  • Akira Matsuno,
  • Mitsuyoshi Sasaki,
  • Mineko Murakami

DOI
https://doi.org/10.5603/4390
Journal volume & issue
Vol. 46, no. 4
pp. 419 – 421

Abstract

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Recently, in order to elucidate the role of rab3B in porosome, we have observed the incorporation of rab3B in the secretion of GH through porosome under confocal laser scanning microscopy (CLSM). Transfected cells with GH-EYFP fusion protein and rab3B-ECFP fusion protein were observed under CLSM, which showed the colocalization of EYFP-GH and ECFP-rab3B in the budding configuration of secretory process. These structural and functional images of rab3B imply the incorporation of rab3B in the secretion of GH through porosome.