Effects of Drying-Rewetting Cycles on Ferrous Iron-Involved Denitrification in Paddy Soils

Abstract

Soil moisture status has an important effect on the process of denitrification in paddy soils. However, it is unclear how it affects the ferrous iron-involved denitrification. Here, the influence of drying-rewetting cycles on ferrous iron-involved denitrification in paddy soil were studied with batch experiments. The dynamics of nitrate, ammonia, Fe2+, Fe3+ and total organic carbon (TOC), as well as nitrous oxide (N2O) were investigated using the iron-rich paddy soil in Jiangxi province, South China. Results demonstrated that the denitrification rate dropped while ammonia nitrogen content (NH4+-N) showed a rapid accumulation in the drying period. In the rewetting period, organic carbon played two-side roles. Organic carbon and ferrous iron together provided electron donors to denitrification, and organic carbon simultaneously reduced ferric iron under anaerobic environment. There were complex interactions among organic carbon, nitrate and Fe2+/Fe3+ under drying-rewetting cycles. Soil rewetting led to denitrification flush, especially after a moderately long drying period, while excessively frequent drying-rewetting alternation was not favorable to nitrate denitrification.

Keywords

• denitrification
• autotrophic denitrification
• iron
• drying and rewetting cycle
• rewetting frequency