Nature Communications (Aug 2020)
Cytokinin fluoroprobe reveals multiple sites of cytokinin perception at plasma membrane and endoplasmic reticulum
- Karolina Kubiasová,
- Juan Carlos Montesinos,
- Olga Šamajová,
- Jaroslav Nisler,
- Václav Mik,
- Hana Semerádová,
- Lucie Plíhalová,
- Ondřej Novák,
- Peter Marhavý,
- Nicola Cavallari,
- David Zalabák,
- Karel Berka,
- Karel Doležal,
- Petr Galuszka,
- Jozef Šamaj,
- Miroslav Strnad,
- Eva Benková,
- Ondřej Plíhal,
- Lukáš Spíchal
Affiliations
- Karolina Kubiasová
- Department of Molecular Biology, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University
- Juan Carlos Montesinos
- Institute of Science and Technology (IST)
- Olga Šamajová
- Department of Cell Biology, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University
- Jaroslav Nisler
- Department of Chemical Biology and Genetics, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University
- Václav Mik
- Department of Chemical Biology and Genetics, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University
- Hana Semerádová
- Institute of Science and Technology (IST)
- Lucie Plíhalová
- Department of Chemical Biology and Genetics, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University
- Ondřej Novák
- Laboratory of Growth Regulators, Institute of Experimental Botany of the Czech Academy of Sciences and Faculty of Science of Palacký University
- Peter Marhavý
- Institute of Science and Technology (IST)
- Nicola Cavallari
- Institute of Science and Technology (IST)
- David Zalabák
- Department of Molecular Biology, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University
- Karel Berka
- Department of Physical Chemistry, Regional Centre of Advanced Technologies and Materials, Faculty of Science, Palacký University
- Karel Doležal
- Department of Chemical Biology and Genetics, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University
- Petr Galuszka
- Jozef Šamaj
- Department of Cell Biology, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University
- Miroslav Strnad
- Laboratory of Growth Regulators, Institute of Experimental Botany of the Czech Academy of Sciences and Faculty of Science of Palacký University
- Eva Benková
- Institute of Science and Technology (IST)
- Ondřej Plíhal
- Department of Molecular Biology, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University
- Lukáš Spíchal
- Department of Chemical Biology and Genetics, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University
- DOI
- https://doi.org/10.1038/s41467-020-17949-0
- Journal volume & issue
-
Vol. 11,
no. 1
pp. 1 – 11
Abstract
Cytokinin receptors predominantly localize to the endoplasmic reticulum. Here, Kubiasová et al. use a cytokinin fluoroprobe to show that ER-localized cytokinin receptors can enter the secretory pathway, reach the plasma membrane and undergo vesicular recycling, suggesting multiple sites of cytokinin perception.
