Potential of Fluoride-Containing Zinc Oxide and Copper Oxide Nanocomposites on Dentin Bonding Ability
Bayarchimeg Altankhishig,
Yasuhiro Matsuda,
Futami Nagano-Takebe,
Katsushi Okuyama,
Hiroko Yamamoto,
Masahiko Sakurai,
Katsuaki Naito,
Mikako Hayashi,
Hidehiko Sano,
Sharanbir K. Sidhu,
Takashi Saito
Affiliations
Bayarchimeg Altankhishig
Division of Clinical Cariology and Endodontology, Department of Oral Rehabilitation, School of Dentistry, Health Sciences University of Hokkaido, Hokkaido 061-0293, Japan
Yasuhiro Matsuda
Division of Clinical Cariology and Endodontology, Department of Oral Rehabilitation, School of Dentistry, Health Sciences University of Hokkaido, Hokkaido 061-0293, Japan
Futami Nagano-Takebe
Department of Dental Materials Science, School of Dentistry, Health Sciences University of Hokkaido, Hokkaido 061-0293, Japan
Katsushi Okuyama
Department of Dental Materials Science, Asahi University School of Dentistry, Mizuho 501-0296, Japan
Hiroko Yamamoto
Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry, Suita 501-0296, Japan
Masahiko Sakurai
Division of Clinical Cariology and Endodontology, Department of Oral Rehabilitation, School of Dentistry, Health Sciences University of Hokkaido, Hokkaido 061-0293, Japan
Katsuaki Naito
Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry, Suita 501-0296, Japan
Mikako Hayashi
Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry, Suita 501-0296, Japan
Hidehiko Sano
Department of Restorative Dentistry, Hokkaido University Graduate School of Dental Medicine, Sapporo 060-8586, Japan
Sharanbir K. Sidhu
Oral Bioengineering, Institute of Dentistry, Queen Mary University of London, London E1 4NS, UK
Takashi Saito
Division of Clinical Cariology and Endodontology, Department of Oral Rehabilitation, School of Dentistry, Health Sciences University of Hokkaido, Hokkaido 061-0293, Japan
Despite recent advances in bonding restorations, which are the basis of restorative dentistry, secondary caries are still able to form. Previously, a novel fluoride-containing zinc and copper (ZCF) nanocomposite was introduced to prevent the formation of caries due to its antibacterial activity. In this study, we studied the impact of ZCF nanoparticles on the adhesive strength of bonding restorations through micro-tensile bond strength (µTBS) testing. The impact of antibacterial and matrix metalloproteinase (MMP) inhibitors on the nanoparticles was also examined. The nanocomposites were prepared using a simple one-step homogeneous co-precipitation method at a low temperature. A self-etch adhesive was applied to 10 extracted caries-free human molars with (test group) and without (control group) the ZCF nanoparticles. This was followed by composite resin build-up and µTBS testing, MMP activity assays, and evaluation of the antibacterial effects. The results showed no significant differences in the µTBS between the ZCF and the control groups. However, the ZCF exhibited a significant inhibitory effect against MMP-2, MMP-8, and MMP-9, in addition to an antibacterial effect on Streptococcus mutans. Therefore, the present study demonstrated that the addition of ZCF nanoparticles to adhesive systems can result in MMP inhibition and antibacterial action while maintaining the mechanical properties of the bonding restorations.
