Mechanism of ribosome rescue by alternative ribosome-rescue factor B

Kai-Hsin Chan; Valentyn Petrychenko; Claudia Mueller; Cristina Maracci; Wolf Holtkamp; Daniel N. Wilson; Niels Fischer; Marina V. Rodnina

doi:10.1038/s41467-020-17853-7

Nature Communications (Aug 2020)

Mechanism of ribosome rescue by alternative ribosome-rescue factor B

Kai-Hsin Chan,
Valentyn Petrychenko,
Claudia Mueller,
Cristina Maracci,
Wolf Holtkamp,
Daniel N. Wilson,
Niels Fischer,
Marina V. Rodnina

Affiliations

Kai-Hsin Chan: Department of Physical Biochemistry, Max Planck Institute for Biophysical Chemistry
Valentyn Petrychenko: Department of Structural Dynamics, Max Planck Institute for Biophysical Chemistry
Claudia Mueller: Institute for Biochemistry and Molecular Biology, University of Hamburg
Cristina Maracci: Department of Physical Biochemistry, Max Planck Institute for Biophysical Chemistry
Wolf Holtkamp: Department of Physical Biochemistry, Max Planck Institute for Biophysical Chemistry
Daniel N. Wilson: Institute for Biochemistry and Molecular Biology, University of Hamburg
Niels Fischer: Department of Structural Dynamics, Max Planck Institute for Biophysical Chemistry
Marina V. Rodnina: Department of Physical Biochemistry, Max Planck Institute for Biophysical Chemistry

DOI: https://doi.org/10.1038/s41467-020-17853-7
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 11

Abstract

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Rescue of ribosomes stalled on non-stop mRNA is essential for cell viability, and several rescue systems to resolve stalling exist in bacteria. Here, the authors use rapid kinetics and cryo-EM to reveal the pathway and selectivity mechanism of ArfB-mediated ribosome rescue.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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