The Clinical Impact of Metagenomic Next-Generation Sequencing (mNGS) Test in Hospitalized Patients with Suspected Sepsis: A Multicenter Prospective Study
Yi-Hui Zuo,
Yi-Xing Wu,
Wei-Ping Hu,
Yan Chen,
Yu-Ping Li,
Zhen-Ju Song,
Zhe Luo,
Min-Jie Ju,
Min-Hua Shi,
Shu-Yun Xu,
Hua Zhou,
Xiang Li,
Zhi-Jun Jie,
Xue-Dong Liu,
Jing Zhang
Affiliations
Yi-Hui Zuo
Department of Pulmonary and Critical Care Medicine, Zhongshan Hospital, Shanghai Medical College, Fudan University, Fenglin Road 180, Xuhui District, Shanghai 200032, China
Yi-Xing Wu
Department of Pulmonary and Critical Care Medicine, Zhongshan Hospital, Shanghai Medical College, Fudan University, Fenglin Road 180, Xuhui District, Shanghai 200032, China
Wei-Ping Hu
Department of Pulmonary and Critical Care Medicine, Zhongshan Hospital, Shanghai Medical College, Fudan University, Fenglin Road 180, Xuhui District, Shanghai 200032, China
Yan Chen
Department of Pulmonary and Critical Care Medicine, Second Xiangya Hospital of Central South University, Mid Renmin Road 139, Changsha 410011, China
Yu-Ping Li
Department of Pulmonary and Critical Care Medicine, The First Affiliated Hospital of Wenzhou Medical University, Fuxue Alley 2, Lucheng District, Wenzhou 325015, China
Zhen-Ju Song
Department of Emergency, Zhongshan Hospital, Shanghai Medical College, Fenglin Road 180, Xuhui District, Shanghai 200032, China
Zhe Luo
Department of Critical Care Medicine, Zhongshan Hospital, Shanghai Medical College, Fudan University, Fenglin Road 180, Xuhui District, Shanghai 200032, China
Min-Jie Ju
Department of Critical Care Medicine, Zhongshan Hospital, Shanghai Medical College, Fudan University, Fenglin Road 180, Xuhui District, Shanghai 200032, China
Min-Hua Shi
Department of Pulmonary Medicine, The Second Affiliated Hospital of Suzhou University, Sanxiang Road 1055, Gusu District, Suzhou 215004, China
Shu-Yun Xu
Department of Pulmonary and Critical Care Medicine, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Jiefang Avenue 1095, Qiaokou District, Wuhan 430030, China
Hua Zhou
Department of Pulmonary and Critical Care Medicine, The First Affiliated Hospital of Zhejiang University, Bunichi West Road 1367, Yuhang District, Hangzhou 310003, China
Xiang Li
Department of Critical Care Medicine, Central Hospital of Minhang District, Shanghai Medical College, Fudan University, Xinsong Road 170, Minhang District, Shanghai 201199, China
Zhi-Jun Jie
Department of Pulmonary and Critical Care Medicine, Shanghai Fifth People’s Hospital, Shanghai Medical College, Fudan University, Ruili Road 128, Minhang District, Shanghai 200240, China
Xue-Dong Liu
Department of Pulmonary and Critical Care Medicine, Qingdao Municipal Hospital, Jiaozhou Road 1, North City District, Qingdao 266071, China
Jing Zhang
Department of Pulmonary and Critical Care Medicine, Zhongshan Hospital, Shanghai Medical College, Fudan University, Fenglin Road 180, Xuhui District, Shanghai 200032, China
Background: Metagenomic Next Generation Sequencing (mNGS) has the potential to detect pathogens rapidly. We aimed to assess the diagnostic performance of mNGS in hospitalized patients with suspected sepsis and evaluate its role in guiding antimicrobial therapy. Methods: A multicenter, prospective cohort study was performed. We enrolled patients with suspected sepsis, collected clinical characteristics and blood samples, and recorded the 30-day survival. Diagnostic efficacy of mNGS test and blood culture was compared, and the clinical impact of mNGS on antibiotic regimen modification was analyzed. Results: A total of 277 patients were enrolled, and 162 were diagnosed with sepsis. The mortality was 44.8% (121/270). The mNGS test exhibited shorter turn-out time (27.0 (26.0, 29.0) vs. 96.0 (72.0, 140.3) hours, p p p = 0.002). Conclusions: Blood mNGS testing might be helpful for early etiological diagnosis of patients with suspected sepsis, guiding the antibiotic regimen modification and improving prognosis.
