LYSOZYME-CONTAINING PREPARATION “ARTIFICIAL TEARS”: OBTAINING, PROPERTIES

Abstract

Aim. The development and study of the chemical and physical properties of eye drops with lysozyme as a perspective drug for the treatment of the syndrome of “dry eye.” Methods. Activity of egg white lysozyme was determined by bacteriolytic method (with Micrococcus lysodeikticus ATCC № 4698 as substrate). Protein content was determined by the Lowry-Hartree method, determination of osmolality of the drug was carried out by osmometer – Tear Lab Osmolarity System. Results. A perspective preparation “artificial tears” for dry eye therapy in form of eye drops with lysozyme was developed. There were noted the complete preservation of bacteriolytic activity of the included enzyme (38200 ± 2000 U/ml). The developed drops have the same values of lacrimal fluid viscosity, density and osmolality. Dextran and hydroxypropylmethyl cellulose stabilized lysozyme in the formulation, with usage of sterilizing filtration stored at low temperatures (0–4 °C) for 12 months with 96.4% retention of the original bacteriolytic activity. Conclusions. We propose a perspective preparation “artificial tears” for dry eye therapy in form of eye drops with lysozyme as a natural antibacterial component of tears. We have noted the high bacteriolytic activity of the included lysozyme (38200 ± 2000 U/ml). It was shown, that developed drops have the same values of lacrimal fluid viscosity, density and osmolality. It is found that dextran stabilized lysozyme in the formulation and hydroxypropylmethylcellulose, with usage sterilizing filtration stored at low temperatures (0–4 °C) for 12 months with 96.4% retention of the original bacteriolytic activity.

Keywords

• лізоцим
• штучна сльоза
• декстран
• гідроксипропілметилцелюлоза
• синдром «сухого ока»