Communications Biology (Apr 2022)

Characterization of Cme and Yme thermostable Cas12a orthologs

  • Ryan T. Fuchs,
  • Jennifer L. Curcuru,
  • Megumu Mabuchi,
  • Audrey Noireterre,
  • Peter R. Weigele,
  • Zhiyi Sun,
  • G. Brett Robb

DOI
https://doi.org/10.1038/s42003-022-03275-2
Journal volume & issue
Vol. 5, no. 1
pp. 1 – 16

Abstract

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Two distinct Cas12a orthologs (Cme and Yme) from metagenomic sources are characterized. The enzymes are found to be thermotolerant and are compared to three mesophilic Cas12a enzymes (Lba, Fno and Asp).