Nuclei isolation from surgically resected human hippocampus

Abstract

Summary: Single-nucleus RNA sequencing (snRNA-seq), where nuclear transcriptomes are a proxy to cellular transcriptomes, has been used to profile human brain. snRNA-seq is sensitive to tissue processing, tissue quality, postmortem interval time, and cellular debris. This protocol outlines steps for the isolation of high-quality nuclei from surgically resected human brain tissue followed by a sucrose gradient yielding neuronal and non-neuronal nuclei enabling unbiased analysis of various cell types.For complete details on the use and execution of this protocol, please refer to Ayhan et al. (2021).

Keywords

• Cell isolation
• Single Cell
• Cell separation/fractionation
• Genomics
• RNAseq
• Microbiology