Nature Communications (Jan 2021)
HIV-1 diversity considerations in the application of the Intact Proviral DNA Assay (IPDA)
- Natalie N. Kinloch,
- Yanqin Ren,
- Winiffer D. Conce Alberto,
- Winnie Dong,
- Pragya Khadka,
- Szu Han Huang,
- Talia M. Mota,
- Andrew Wilson,
- Aniqa Shahid,
- Don Kirkby,
- Marianne Harris,
- Colin Kovacs,
- Erika Benko,
- Mario A. Ostrowski,
- Perla M. Del Rio Estrada,
- Avery Wimpelberg,
- Christopher Cannon,
- W. David Hardy,
- Lynsay MacLaren,
- Harris Goldstein,
- Chanson J. Brumme,
- Guinevere Q. Lee,
- Rebecca M. Lynch,
- Zabrina L. Brumme,
- R. Brad Jones
Affiliations
- Natalie N. Kinloch
- Faculty of Health Sciences, Simon Fraser University
- Yanqin Ren
- Infectious Diseases Division, Department of Medicine, Weill Cornell Medical College
- Winiffer D. Conce Alberto
- Infectious Diseases Division, Department of Medicine, Weill Cornell Medical College
- Winnie Dong
- British Columbia Centre for Excellence in HIV/AIDS
- Pragya Khadka
- Infectious Diseases Division, Department of Medicine, Weill Cornell Medical College
- Szu Han Huang
- Infectious Diseases Division, Department of Medicine, Weill Cornell Medical College
- Talia M. Mota
- Infectious Diseases Division, Department of Medicine, Weill Cornell Medical College
- Andrew Wilson
- Department of Microbiology, Immunology and Tropical Medicine, George Washington University
- Aniqa Shahid
- Faculty of Health Sciences, Simon Fraser University
- Don Kirkby
- British Columbia Centre for Excellence in HIV/AIDS
- Marianne Harris
- British Columbia Centre for Excellence in HIV/AIDS
- Colin Kovacs
- Maple Leaf Medical Clinic
- Erika Benko
- Maple Leaf Medical Clinic
- Mario A. Ostrowski
- Department of Medicine, University of Toronto
- Perla M. Del Rio Estrada
- Center for Research in Infectious Diseases, National Institute of Respiratory Diseases
- Avery Wimpelberg
- Whitman Walker Health
- Christopher Cannon
- Whitman Walker Health
- W. David Hardy
- Whitman Walker Health
- Lynsay MacLaren
- Whitman Walker Health
- Harris Goldstein
- Department of Microbiology and Immunology, Albert Einstein College of Medicine
- Chanson J. Brumme
- British Columbia Centre for Excellence in HIV/AIDS
- Guinevere Q. Lee
- Infectious Diseases Division, Department of Medicine, Weill Cornell Medical College
- Rebecca M. Lynch
- Department of Microbiology, Immunology and Tropical Medicine, George Washington University
- Zabrina L. Brumme
- Faculty of Health Sciences, Simon Fraser University
- R. Brad Jones
- Infectious Diseases Division, Department of Medicine, Weill Cornell Medical College
- DOI
- https://doi.org/10.1038/s41467-020-20442-3
- Journal volume & issue
-
Vol. 12,
no. 1
pp. 1 – 10
Abstract
The intact proviral DNA assay quantifies the genomically intact HIV reservoir, but assay failure due to HIV-1 polymorphism has been observed. Here, the authors report a 28% failure rate in a cohort of people with HIV-1, and note within-host HIV-1 diversity as a further challenge to IPDA accuracy.
