OncoTargets and Therapy (Jan 2020)
Expression and Effects of Long Non-Coding RNA, LINC01124, in Non-Small Cell Lung Cancer
Abstract
Zi-Bo Wang, Hong-Yan Zhang, Ji-Bin Lu Department of Thoracic Surgery, Shengjing Hospital of China Medical University, Shenyang 110000, People’s Republic of ChinaCorrespondence: Ji-Bin LuDepartment of Thoracic Surgery, Shengjing Hospital of China Medical University, Shenyang 110000, People’s Republic of ChinaTel +86 18940251178Email [email protected]: To investigate the expression and evaluate the clinical significance of long non-coding RNA, LINC01124, in non-small cell lung cancer (NSCLC) and to study its influence in this tumor.Methods: Hundred specimens of NSCLC tissues and normal lung tissues after surgery were collected. The qRT-PCR for LINC01124 expression was performed on cancerous and normal lung tissues. The correlations between the expression of LINC01124 and pathological characteristics were analyzed. PcDNA-LINC01124 was transfected to upregulate LINC01124 expression in NSCLC cells, and the transfection efficiency was evaluated by the qRT-PCR. CCK8 assay, wound-healing assay, and the Transwell assay were performed to evaluate the effect of ectopic LINC01124 expression on proliferation, migration, and invasive of NSCLC cells.Results: The expression level of LINC01124 was downregulated in tumor tissues when compared with the paired normal lung tissues (P<0.05). The expression of LINC01124 was associated with patients’ age and distant metastasis (P<0.05). Enforced expression of LINC01124 significantly inhibited the proliferation, migration, and invasive ability of NSCLC cells.Conclusion: The expression of LINC01124 was decreased in patients with NSCLC of older age and with those having distant metastasis. LINC01124 may inhibit cell proliferation, migration, and invasive ability.Keywords: non-small cell lung cancer, NSCLC, long non-coding RNA, lncRNA, LINC01124
