Programmed spatial organization of biomacromolecules into discrete, coacervate-based protocells

Wiggert J. Altenburg; N. Amy Yewdall; Daan F. M. Vervoort; Marleen H. M. E. van Stevendaal; Alexander F. Mason; Jan C. M. van Hest

doi:10.1038/s41467-020-20124-0

Nature Communications (Dec 2020)

Programmed spatial organization of biomacromolecules into discrete, coacervate-based protocells

Wiggert J. Altenburg,
N. Amy Yewdall,
Daan F. M. Vervoort,
Marleen H. M. E. van Stevendaal,
Alexander F. Mason,
Jan C. M. van Hest

Affiliations

Wiggert J. Altenburg: Department of Biomedical Engineering, Eindhoven University of Technology
N. Amy Yewdall: Department of Biomedical Engineering, Eindhoven University of Technology
Daan F. M. Vervoort: Department of Biomedical Engineering, Eindhoven University of Technology
Marleen H. M. E. van Stevendaal: Institute for Complex Molecular Systems, Eindhoven University of Technology
Alexander F. Mason: Institute for Complex Molecular Systems, Eindhoven University of Technology
Jan C. M. van Hest: Department of Biomedical Engineering, Eindhoven University of Technology

DOI: https://doi.org/10.1038/s41467-020-20124-0
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 10

Abstract

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Mimicking the crowded cytosol of cells in synthetic cells has been a major limitation to the functionality. Here, the authors used the interaction between nickel, nitrilotriacetic acid and histidine tagged proteins to control loading of macromolecules into spatially programmed coacervate-based protocells.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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