Frontiers in Energy Research (Dec 2021)
Isolation and Characterization of Bacillus Sp. Capable of Degradating Alkali Lignin
- Jing Yang,
- Jing Yang,
- Jing Yang,
- Jing Yang,
- Jing Yang,
- Jian Zhao,
- Jian Zhao,
- Jian Zhao,
- Jian Zhao,
- Jian Zhao,
- Jianchun Jiang,
- Jianchun Jiang,
- Jianchun Jiang,
- Jianchun Jiang,
- Jianchun Jiang,
- Hao Xu,
- Hao Xu,
- Hao Xu,
- Hao Xu,
- Hao Xu,
- Ning Zhang,
- Ning Zhang,
- Ning Zhang,
- Ning Zhang,
- Ning Zhang,
- Jingcong Xie,
- Jingcong Xie,
- Jingcong Xie,
- Jingcong Xie,
- Jingcong Xie,
- Min Wei,
- Min Wei,
- Min Wei,
- Min Wei,
- Min Wei
Affiliations
- Jing Yang
- Institute of Chemical Industry of Forest Products, CAF, Nanjing, China
- Jing Yang
- National Engineering Laboratory for Biomass Chemical Utilization, Nanjing, China
- Jing Yang
- Key and Open Laboratory of Forest Chemical Engineering, SFA, Nanjing, China
- Jing Yang
- Key Laboratory of Biomass Energy and Material, Nanjing, China
- Jing Yang
- Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing, China
- Jian Zhao
- Institute of Chemical Industry of Forest Products, CAF, Nanjing, China
- Jian Zhao
- National Engineering Laboratory for Biomass Chemical Utilization, Nanjing, China
- Jian Zhao
- Key and Open Laboratory of Forest Chemical Engineering, SFA, Nanjing, China
- Jian Zhao
- Key Laboratory of Biomass Energy and Material, Nanjing, China
- Jian Zhao
- Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing, China
- Jianchun Jiang
- Institute of Chemical Industry of Forest Products, CAF, Nanjing, China
- Jianchun Jiang
- National Engineering Laboratory for Biomass Chemical Utilization, Nanjing, China
- Jianchun Jiang
- Key and Open Laboratory of Forest Chemical Engineering, SFA, Nanjing, China
- Jianchun Jiang
- Key Laboratory of Biomass Energy and Material, Nanjing, China
- Jianchun Jiang
- Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing, China
- Hao Xu
- Institute of Chemical Industry of Forest Products, CAF, Nanjing, China
- Hao Xu
- National Engineering Laboratory for Biomass Chemical Utilization, Nanjing, China
- Hao Xu
- Key and Open Laboratory of Forest Chemical Engineering, SFA, Nanjing, China
- Hao Xu
- Key Laboratory of Biomass Energy and Material, Nanjing, China
- Hao Xu
- Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing, China
- Ning Zhang
- Institute of Chemical Industry of Forest Products, CAF, Nanjing, China
- Ning Zhang
- National Engineering Laboratory for Biomass Chemical Utilization, Nanjing, China
- Ning Zhang
- Key and Open Laboratory of Forest Chemical Engineering, SFA, Nanjing, China
- Ning Zhang
- Key Laboratory of Biomass Energy and Material, Nanjing, China
- Ning Zhang
- Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing, China
- Jingcong Xie
- Institute of Chemical Industry of Forest Products, CAF, Nanjing, China
- Jingcong Xie
- National Engineering Laboratory for Biomass Chemical Utilization, Nanjing, China
- Jingcong Xie
- Key and Open Laboratory of Forest Chemical Engineering, SFA, Nanjing, China
- Jingcong Xie
- Key Laboratory of Biomass Energy and Material, Nanjing, China
- Jingcong Xie
- Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing, China
- Min Wei
- Institute of Chemical Industry of Forest Products, CAF, Nanjing, China
- Min Wei
- National Engineering Laboratory for Biomass Chemical Utilization, Nanjing, China
- Min Wei
- Key and Open Laboratory of Forest Chemical Engineering, SFA, Nanjing, China
- Min Wei
- Key Laboratory of Biomass Energy and Material, Nanjing, China
- Min Wei
- Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing, China
- DOI
- https://doi.org/10.3389/fenrg.2021.807286
- Journal volume & issue
-
Vol. 9
Abstract
Alkali lignin-degrading Bacillus were isolated from forest soils in China and were identified as Bacillus subtilis TR-03 and Bacillus cereus TR-25 by 16S rDNA sequence analysis. Wherein TR-03 displayed optimal 26.72% alkali lignin (2 g/L) degradation at 7 days and 71.23% of Azure-B (0.01%) decolorization at 36 h of cultivation at 37°C. Ligninolytic enzyme analysis revealed that TR-03 was capable of depolymerizing alkali lignin effectively by the producing of lignin peroxidase and laccase, wherein higher laccase activity was cell-associated. At last, the physical and chemical changes of lignin via SEM and FTIR analysis was further observed to prove the lignin degradation by Bacillus subtilis TR-03.
Keywords
