Nature Communications (Aug 2019)

Maximizing binary interactome mapping with a minimal number of assays

  • Soon Gang Choi,
  • Julien Olivet,
  • Patricia Cassonnet,
  • Pierre-Olivier Vidalain,
  • Katja Luck,
  • Luke Lambourne,
  • Kerstin Spirohn,
  • Irma Lemmens,
  • Mélanie Dos Santos,
  • Caroline Demeret,
  • Louis Jones,
  • Sudharshan Rangarajan,
  • Wenting Bian,
  • Eloi P. Coutant,
  • Yves L. Janin,
  • Sylvie van der Werf,
  • Philipp Trepte,
  • Erich E. Wanker,
  • Javier De Las Rivas,
  • Jan Tavernier,
  • Jean-Claude Twizere,
  • Tong Hao,
  • David E. Hill,
  • Marc Vidal,
  • Michael A. Calderwood,
  • Yves Jacob

DOI
https://doi.org/10.1038/s41467-019-11809-2
Journal volume & issue
Vol. 10, no. 1
pp. 1 – 13

Abstract

Read online

Comprehensive mapping of binary protein-protein interactions requires to combine several complementary assays. Here, the authors show that complete coverage could be reached with a minimal number of assays as long as they explore various experimental conditions.