Effect of different culture media on the growth and antifungal activity ofBacillus pumilus CCIBP-C5

Abstract

Plantains and bananas (Musa spp.) are crops that require abundant fertilization and are affectedby numerous pests and diseases, including Black Sigatoka. Previous studies demonstrated thepotential of theBacillus pumilus strain CCIBP-C5 for thein vitro and greenhouse control ofPseudocercospora fijiensis Morelet and to stimulate the growth ofMusa spp. Several studiesare still required to develop a bioproduct based on this strain. For this reason, this researchaims to determine the effect of different culture media on the growth and antifungal activityofBacillus pumilus CCIBP-C5. Two culture media made with industrial by-products, in whichthe growth kinetics of the strain and the effect of culture filtrates (FC) on the growthinhibition ofPseudocercospora fijiensis we re evaluate d. The bacterial strain was able to g rowin both culture media and inhibition of fungal growth was observed in all FCs. The FC obtainedin Soy-Chitin Broth produced greater growth inhibition compared to the FC obtained in Soy- Molasses Broth. It is concluded that the Soy Broth-Chitin culture medium could be the mostindicated for the elaboration of a bioproduct fromB. pumilus CCIBP-C5

Keywords

• bioproduct
• biological control
• fermentation
• pseudocercospora fijiensis