International Journal of Molecular Sciences (Nov 2015)

MiR542-3p Regulates the Epithelial-Mesenchymal Transition by Directly Targeting BMP7 in NRK52e

  • Zhicheng Liu,
  • Yuru Zhou,
  • Yue Yuan,
  • Fang Nie,
  • Rui Peng,
  • Qianyin Li,
  • Zhongshi Lyu,
  • Zhaomin Mao,
  • Liyuan Huang,
  • Li Zhou,
  • Yiman Li,
  • Jing Hao,
  • Dongsheng Ni,
  • Qianni Jin,
  • Yaoshui Long,
  • Pan Ju,
  • Wen Yu,
  • Jianing Liu,
  • Yanxia Hu,
  • Qin Zhou

Journal volume & issue
Vol. 16, no. 11
pp. 27945 – 27955


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Accumulating evidence demonstrated that miRNAs are highly involved in kidney fibrosis and Epithelial-Eesenchymal Transition (EMT), however, the mechanisms of miRNAs in kidney fibrosis are poorly understood. In this work, we identified that miR542-3p could promote EMT through down-regulating bone morphogenetic protein 7 (BMP7) expression by targeting BMP7 3′UTR. Firstly, real-time PCR results showed that miR542-3p was significantly up-regulated in kidney fibrosis in vitro and in vivo. Moreover, Western blot results demonstrated that miR542-3p may promote EMT in the NRK52e cell line. In addition, we confirmed that BMP7, which played a crucial role in anti-kidney fibrosis and suppressed the progression of EMT, was a target of miR542-3p through Dual-Luciferase reporter assay, as did Western blot analysis. The effects of miR542-3p on regulating EMT could also be suppressed by transiently overexpressing BMP7 in NRK52e cells. Taken together, miR542-3p may be a critical mediator of the induction of EMT via directly targeting BMP7.