Maize–Soybean Rotation and Intercropping Increase Maize Yield by Influencing the Structure and Function of Rhizosphere Soil Fungal Communities

Abstract

Soil-borne diseases are exacerbated by continuous cropping and negatively impact maize health and yields. We conducted a long-term (11-year) field experiment in the black soil region of Northeast China to analyze the effects of different cropping systems on maize yield and rhizosphere soil fungal community structure and function. The experiment included three cropping systems: continuous maize cropping (CMC), maize–soybean rotation (MSR), and maize–soybean intercropping (MSI). MSI and MSR resulted in a 3.30–16.26% lower ear height coefficient and a 7.43–12.37% higher maize yield compared to CMC. The richness and diversity of rhizosphere soil fungi were 7.75–20.26% lower in MSI and MSR than in CMC. The relative abundances of Tausonia and Mortierella were associated with increased maize yield, whereas the relative abundance of Solicoccozyma was associated with decreased maize yield. MSI and MSR had higher proportions of wood saprotrophs and lower proportions of plant pathogens than CMC. Furthermore, our findings indicate that crop rotation is more effective than intercropping for enhancing maize yield and mitigating soil-borne diseases in the black soil zone of Northeast China. This study offers valuable insights for the development of sustainable agroecosystems.

Keywords

• continuous maize cropping
• maize–soybean rotation
• maize–soybean intercropping
• fungal community diversity
• fungal community function
• soil-borne diseases