Morphological damage and increased ROS production of biosynthesized silver nanoparticle against MCF-7 breast cancer cells through in vitro approaches

Abstract

In this study of anti-cancer activity, the cytotoxicity effect of the silver nanoparticle was shown excellent anti-cancer activity against MCF-7 breast cancer cells. The IC50 concentration of the synthesized silver nanoparticle against performed MCF-7 breast cancer cells was 1000 µg/ml concentration. Further, the shape modifications in the silver nanoparticle treated MCF-7 cells were viewed by phase contrast microscope and proved after comparison with untreated control. Next, the intracellular proliferation, chromatin condensation, granular membranes cleavage and surface layers damage were effectively seen by fluorescence microscopy using AO/EB fluorescence dyes. The failure of matured nucleus was clearly shown after the treatment of Ag NPs and was effectively proved by fluorescence microscope using Hoechst 33342 stain. Finally, the anti-cancer effect identification through various invitro experiments was clearly favored to performed silver nanoparticle, and suggested that the nanoparticle was very effective against multi drug resistant bacteria, biofilm producing bacteria and cancer cells.

Keywords

• Silver nanoparticle
• Anticancer activity
• MCF-7 breast cancer cells
• Cytotoxicity effect
• Morphological modification
• Fluorescence microscope