Molecular typing of 151 strains of Vibrio parahaemolyticus

Abstract

ObjectiveTo provide a basis for the tracing and early warning of foodborne diseases caused by Vibrio parahaemolyticus （VP） in Liaoning Province， the distribution and epidemic trend of VP in Liaoning Province combined with serotype analysis were elucidated.MethodsOne hundred and fifty-one VP isolates from Liaoning Province in 2019 were serotyped， and PFGE， REP-PCR， and ERIC-PCR molecular typing and cluster analysis were performed to evaluate the correlation between the three methods and explore the kinship between the strains.ResultsOne hundred and twenty-four isolates were divided into 18 serotypes and 27 isolates could not be classified. The main serotypes were the O3， O1， and O2 groups. The resolutions （DI） of PFGE， REP-PCR， and ERIC-PCR were 0.97， 0.95， and 0.93， respectively. There was a higher similarity in the molecular type between the serotype O3 group strain and the O1 group strain.ConclusionThe epidemic serogroup of clinical VP isolates in Liaoning Province is still O3：K6， and the epidemic serogroup of food VP isolates is still O2. The cluster analysis results of PFGE， REP-PCR， and ERIC-PCR are consistent， and the resolution and reproducibility of PFGE are better than those of the other two methods. These results can provide novel technical means for the traceability of foodborne diseases caused by VP.

Keywords

• vibrio parahaemolyticus
• molecular typing
• serum typing
• kinship
• traceability