Generation of Parkinson’s disease patient-derived human induced pluripotent stem cells line (PNUSCRi001-A) carrying a N227S mutation in GBA gene

Nayeon Lee; Hye-Ji Moon; So-Hyun Park; Ji-Young Moon; Ka-Kyung Park; Jae-Ho Kim; Jae-Hyeok Lee

Stem Cell Research (Dec 2022)

Generation of Parkinson’s disease patient-derived human induced pluripotent stem cells line (PNUSCRi001-A) carrying a N227S mutation in GBA gene

Nayeon Lee,
Hye-Ji Moon,
So-Hyun Park,
Ji-Young Moon,
Ka-Kyung Park,
Jae-Ho Kim,
Jae-Hyeok Lee

Affiliations

Nayeon Lee: Convergence Stem Cell Research Center, Medical Research Institute, Pusan National University School of Medicine, Yangsan, Gyeongsangnam-do, Republic of Korea; Department of Physiology, Pusan National University School of Medicine, Yangsan, Gyeongsangnam-do, Republic of Korea
Hye-Ji Moon: Convergence Stem Cell Research Center, Medical Research Institute, Pusan National University School of Medicine, Yangsan, Gyeongsangnam-do, Republic of Korea; Department of Physiology, Pusan National University School of Medicine, Yangsan, Gyeongsangnam-do, Republic of Korea
So-Hyun Park: Department of Neurology, Research Institute for Convergence of Biomedical Science and Technology, Pusan National University Yangsan Hospital, Yangsan, Gyeongsangnam-do, Republic of Korea
Ji-Young Moon: Convergence Stem Cell Research Center, Medical Research Institute, Pusan National University School of Medicine, Yangsan, Gyeongsangnam-do, Republic of Korea; Department of Physiology, Pusan National University School of Medicine, Yangsan, Gyeongsangnam-do, Republic of Korea
Ka-Kyung Park: Convergence Stem Cell Research Center, Medical Research Institute, Pusan National University School of Medicine, Yangsan, Gyeongsangnam-do, Republic of Korea; Department of Physiology, Pusan National University School of Medicine, Yangsan, Gyeongsangnam-do, Republic of Korea
Jae-Ho Kim: Convergence Stem Cell Research Center, Medical Research Institute, Pusan National University School of Medicine, Yangsan, Gyeongsangnam-do, Republic of Korea; Department of Physiology, Pusan National University School of Medicine, Yangsan, Gyeongsangnam-do, Republic of Korea
Jae-Hyeok Lee: Department of Neurology, Research Institute for Convergence of Biomedical Science and Technology, Pusan National University Yangsan Hospital, Yangsan, Gyeongsangnam-do, Republic of Korea; Corresponding author.

Journal volume & issue: Vol. 65
p. 102959

Abstract

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The hiPSC line was generated from peripheral blood mononuclear cells (PBMCs) collected by a male patient with young onset Parkinson’s disease, carrying on heterozygous c.680 A > G (N227S) mutation in the GBA gene. The PBMCs was reprogrammed into an induced pluripotent stem cell (iPSC) line (PNUSCRi001-A hiPSCs) using non-integrative sendai virus. The hiPSC line, PNUSCRi001-A displayed a normal karyotype and the Expression of pluripotency markers that is capable of producing derivatives of three germ layers (Ectoderm, Endoderm and Mesoderm).

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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