Antitumor Effect of Epigallocatechin Gallate and Vincristine in Mice with L5178Y Lymphoma
Georgina Almaguer,
Gustavo Almaguer-Vargas,
Eva María Molina-Trinidad,
Marco Antonio Becerril-Flores,
Brenda Montejano,
Eduardo Madrigal-Santillan,
Alejandra Hernández-Ceruelos,
Ana Hilda Figueroa-Gutiérrez,
Ethoan Montejano,
José Ramón Montejano-Rodríguez
Affiliations
Georgina Almaguer
Institute of Health Sciences, Autonomous University of the State of Hidalgo, Ex-Hacienda de la Concepción, Tilcuautla 42183, Mexico
Gustavo Almaguer-Vargas
Plant Breeding Department, Horticulture Institute, Chapingo Autonomous University, Federal Highway Mexico-Texcoco km 38.5, Chapingo, Texcoco 56230, Mexico
Eva María Molina-Trinidad
Institute of Health Sciences, Autonomous University of the State of Hidalgo, Ex-Hacienda de la Concepción, Tilcuautla 42183, Mexico
Marco Antonio Becerril-Flores
Institute of Health Sciences, Autonomous University of the State of Hidalgo, Ex-Hacienda de la Concepción, Tilcuautla 42183, Mexico
Brenda Montejano
Department of Pharmacy, Hospital San José, Santiago de Querétaro 76180, Mexico
Eduardo Madrigal-Santillan
Institute of Health Sciences, Autonomous University of the State of Hidalgo, Ex-Hacienda de la Concepción, Tilcuautla 42183, Mexico
Alejandra Hernández-Ceruelos
Institute of Health Sciences, Autonomous University of the State of Hidalgo, Ex-Hacienda de la Concepción, Tilcuautla 42183, Mexico
Ana Hilda Figueroa-Gutiérrez
Institute of Health Sciences, Autonomous University of the State of Hidalgo, Ex-Hacienda de la Concepción, Tilcuautla 42183, Mexico
Ethoan Montejano
Interdisciplinary Professional Unit of Biotechnology IPN, National Polytechnic Institute, Av. Acueducto, La Laguna Ticoman, Gustavo A. Madero, Mexico City 07340, Mexico
José Ramón Montejano-Rodríguez
Institute of Health Sciences, Autonomous University of the State of Hidalgo, Ex-Hacienda de la Concepción, Tilcuautla 42183, Mexico
The main objective of research into new therapies is the search for more efficacy and fewer toxic effects in cancer treatments. On one hand, vincristine (VCR) is a chemotherapeutic used in different kinds of tumors. On the other hand, epigallocatechin gallate (EGCG) is a green tea metabolite that has shown an antineoplastic effect in diverse investigations, so the objective of this work is to evaluate the antitumor effects of the EGCG/VCR combination on tumor volume and survival. To achieve this objective, the solid model of lymphoma L5178Y was used in BALB/c mice with different doses of VCR, EGCG, and their combination allowed tumor growth and survival time recording. After tumor collection, measurements, and immunohistochemistry for p53, Bcl2, and Cyclin D1 were performed. The results showed that the EGCG/vincristine combination had a greater antitumor effect than those effects of vincristine and EGCG. It can be attributed to the fact that the greatest inhibition of Bcl2 was present in gathering of EGCG harvest with vincristine. Therefore, the combination of EGCG with vincristine has a better antineoplastic effect by inhibiting tumor development and increasing survival on both substances independently.
