Genotyping Lupinus angustifolius cultivars with SRAP molecular markers and degenerate primers

Sysoliatin Eugeny Nikolaevich; Anisimova Natalia Vladimirovna; Babak Olga Gennadievna

doi:10.24190/ISSN2564-615X/2017/02.18

The EuroBiotech Journal (May 2017)

Genotyping Lupinus angustifolius cultivars with SRAP molecular markers and degenerate primers

Sysoliatin Eugeny Nikolaevich,
Anisimova Natalia Vladimirovna,
Babak Olga Gennadievna

Affiliations

Sysoliatin Eugeny Nikolaevich: Laboratory of Ecological Genetics and Biotechnology, Institute of Genetics and Cytology of NAS of Belarus, Minsk, Belarus
Anisimova Natalia Vladimirovna: Laboratory of Ecological Genetics and Biotechnology, Institute of Genetics and Cytology of NAS of Belarus, Minsk, Belarus
Babak Olga Gennadievna: Laboratory of Ecological Genetics and Biotechnology, Institute of Genetics and Cytology of NAS of Belarus, Minsk, Belarus

DOI: https://doi.org/10.24190/ISSN2564-615X/2017/02.18
Journal volume & issue: Vol. 1, no. 2
pp. 200 – 201

Abstract

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We examined 18 combinations of SRAP primers with resistance gene analog (RGA) and chitinase degenerate primers in order to determine their utility for genotyping L. angustifolius. Primer pairs ResAn51-f/Me8, p-loop/Em5, TM/Me8, Chit3-r/Em5 were the most effective for detection of genetic polymorphism of different narrow-leaved lupine varieties.

Published in The EuroBiotech Journal

ISSN: 2564-615X (Online)
Publisher: Sciendo
Country of publisher: Poland
LCC subjects: Technology: Chemical technology: Biotechnology
Website: https://sciendo.com/journal/EBTJ

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