Isolation and characterization of mesenchymal stem cells obtained from reusable and disposable bone marrow collection filters

Abstract

Objective: To compare human mesenchymal stem cells obtainedfrom reusable and disposable filters and to characterize themaccording to the criteria of the International Society of CellularTherapy. Methods: Human mesenchymal stem cells were isolatedfrom bone marrow collection reusable sets and compared with thoseobtained from disposable sets by washing the filters with cell culturemedia. The isolated cells were characterized according to the criteriaof the International Society of Cellular Therapy using flow cytometry,differentiation in vitro, and cytochemistry techniques. Results:Samples were obtained from disposable (n=3) and from reusablecollection sets (n=3). All samples obtained from bone marrowdisposable sets successfully produced mesenchymal stem cells. Allbone marrow derived mesenchymal stem cells were characterizedand fulfilled the criteria established by International Society of Cellular Therapy. Conclusion: This study showed that mesenchymal stemcells can also be obtained from reusable collection sets (which arestill used in several centers around the world) to be employed inresearch as an alternative and ethical source.

Keywords

• Mesenchymal stem cells
• Bone marrow
• Filtration
• Disposable equipment
• Recycling