CRISPR-Cas9/Cas12a biotechnology and application in bacteria

Ruilian Yao; Di Liu; Xiao Jia; Yuan Zheng; Wei Liu; Yi Xiao

Synthetic and Systems Biotechnology (Sep 2018)

CRISPR-Cas9/Cas12a biotechnology and application in bacteria

Ruilian Yao,
Di Liu,
Xiao Jia,
Yuan Zheng,
Wei Liu,
Yi Xiao

Affiliations

Ruilian Yao: State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China
Di Liu: Department of Biomass Science and Conversion Technology, Sandia National Laboratories, Livermore, CA 94551, USA
Xiao Jia: State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China
Yuan Zheng: State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China
Wei Liu: State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China
Yi Xiao: State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China; Corresponding author.

Journal volume & issue: Vol. 3, no. 3
pp. 135 – 149

Abstract

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CRISPR-Cas technologies have greatly reshaped the biology field. In this review, we discuss the CRISPR-Cas with a particular focus on the associated technologies and applications of CRISPR-Cas9 and CRISPR-Cas12a, which have been most widely studied and used. We discuss the biological mechanisms of CRISPR-Cas as immune defense systems, recently-discovered anti-CRISPR-Cas systems, and the emerging Cas variants (such as xCas9 and Cas13) with unique characteristics. Then, we highlight various CRISPR-Cas biotechnologies, including nuclease-dependent genome editing, CRISPR gene regulation (including CRISPR interference/activation), DNA/RNA base editing, and nucleic acid detection. Last, we summarize up-to-date applications of the biotechnologies for synthetic biology and metabolic engineering in various bacterial species. Keywords: Cas9, Cas12a (Cpf1), Cas13, Base editing, DNA/RNA detection

Published in Synthetic and Systems Biotechnology

ISSN: 2405-805X (Online)
Publisher: KeAi Communications Co., Ltd.
Country of publisher: China
LCC subjects: Technology: Chemical technology: Biotechnology; Science: Biology (General)
Website: https://www.keaipublishing.com/en/journals/synthetic-and-systems-biotechnology/

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