International Journal of Nanomedicine (Nov 2020)

Surface-Engineered Cubosomes Serve as a Novel Vaccine Adjuvant to Modulate Innate Immunity and Improve Adaptive Immunity in vivo

  • Liu Z,
  • Yu L,
  • Gu P,
  • Bo R,
  • Xu S,
  • Wusiman A,
  • Liu J,
  • Hu Y,
  • Wang D

Journal volume & issue
Vol. Volume 15
pp. 8595 – 8608

Abstract

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Zhenguang Liu,1,2 Lin Yu,1,2 Pengfei Gu,1,2 Ruonan Bo,1,2 Shuwen Xu,1,2 Adelijiang Wusiman,1,2 Jiaguo Liu,1,2 Yuanliang Hu,1,2 Deyun Wang1,2 1Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, People’s Republic of China; 2MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, People’s Republic of ChinaCorrespondence: Deyun WangInstitute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, People’s Republic of ChinaTel +86-25-84395203Fax +86-25-84398669Email [email protected]: Recent studies have revealed the adjuvant activity of cubosomes and their potential utility as an antigen delivery system. In this study, to further enhance the adjuvant activity of cubosomes, two cationic polymers are modified on the surface of cubosomes.Methods: Here, we exploit the effects of surface chemistry on the adjuvant activity of Ganoderma lucidum polysaccharide cubosomes (GLPC) by placing two kinds of molecules, that is, cetyltrimethylammonium bromide (CTAB) and poly(diallydimethyl ammonium chloride) (PDDAC), on their surface.Results: CTAB- or PDDAC-modified GLPC were found to significantly promote humoral and cellular immune responses, as well as the proliferation of CD3+ CD4+ or CD3+ CD8+T cells through the powerful activation of dendritic cells (DCs). The enhanced immune responses of PDDAC-modified GLPC might be attributed to the maturation of DCs into draining lymph nodes and the activation of spleen and cytokines in serum.Conclusion: PDDAC modification is beneficial for enhancing humoral and cellular immune response, suggesting that PDDAC-GLPC-OVA has the ability to be a potential adjuvant for vaccine.Keywords: cubosomes, Ganoderma lucidum polysaccharide, surface-engineering, immune response, RNA-seq

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