Frontiers in Plant Science (Apr 2022)
Rosa1, a Transposable Element-Like Insertion, Produces Red Petal Coloration in Rose Through Altering RcMYB114 Transcription
- Maofu Li,
- Maofu Li,
- Maofu Li,
- Hui Zhang,
- Hui Zhang,
- Yuan Yang,
- Yuan Yang,
- Yuan Yang,
- Hua Wang,
- Hua Wang,
- Hua Wang,
- Zhen Xue,
- Zhen Xue,
- Youwei Fan,
- Youwei Fan,
- Youwei Fan,
- Pei Sun,
- Pei Sun,
- Pei Sun,
- Hong Zhang,
- Hong Zhang,
- Hong Zhang,
- Xinzhu Zhang,
- Xinzhu Zhang,
- Xinzhu Zhang,
- Wanmei Jin,
- Wanmei Jin,
- Wanmei Jin
Affiliations
- Maofu Li
- Institute of Forestry and Pomology, Beijing Academy of Agriculture and Forestry Sciences, Beijing, China
- Maofu Li
- Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Ministry of Agriculture and Rural Affairs, Beijing, China
- Maofu Li
- Beijing Engineering Research Center of Functional Floriculture, Beijing, China
- Hui Zhang
- Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, China
- Hui Zhang
- Institute of Botany, University of Chinese Academy of Sciences, Beijing, China
- Yuan Yang
- Institute of Forestry and Pomology, Beijing Academy of Agriculture and Forestry Sciences, Beijing, China
- Yuan Yang
- Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Ministry of Agriculture and Rural Affairs, Beijing, China
- Yuan Yang
- Beijing Engineering Research Center for Deciduous Fruit Trees, Beijing, China
- Hua Wang
- Institute of Forestry and Pomology, Beijing Academy of Agriculture and Forestry Sciences, Beijing, China
- Hua Wang
- Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Ministry of Agriculture and Rural Affairs, Beijing, China
- Hua Wang
- Beijing Engineering Research Center of Functional Floriculture, Beijing, China
- Zhen Xue
- Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, China
- Zhen Xue
- Institute of Botany, University of Chinese Academy of Sciences, Beijing, China
- Youwei Fan
- Institute of Forestry and Pomology, Beijing Academy of Agriculture and Forestry Sciences, Beijing, China
- Youwei Fan
- Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Ministry of Agriculture and Rural Affairs, Beijing, China
- Youwei Fan
- Beijing Engineering Research Center of Functional Floriculture, Beijing, China
- Pei Sun
- Institute of Forestry and Pomology, Beijing Academy of Agriculture and Forestry Sciences, Beijing, China
- Pei Sun
- Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Ministry of Agriculture and Rural Affairs, Beijing, China
- Pei Sun
- Beijing Engineering Research Center of Functional Floriculture, Beijing, China
- Hong Zhang
- Institute of Forestry and Pomology, Beijing Academy of Agriculture and Forestry Sciences, Beijing, China
- Hong Zhang
- Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Ministry of Agriculture and Rural Affairs, Beijing, China
- Hong Zhang
- Beijing Engineering Research Center of Functional Floriculture, Beijing, China
- Xinzhu Zhang
- Institute of Forestry and Pomology, Beijing Academy of Agriculture and Forestry Sciences, Beijing, China
- Xinzhu Zhang
- Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Ministry of Agriculture and Rural Affairs, Beijing, China
- Xinzhu Zhang
- Beijing Engineering Research Center of Functional Floriculture, Beijing, China
- Wanmei Jin
- Institute of Forestry and Pomology, Beijing Academy of Agriculture and Forestry Sciences, Beijing, China
- Wanmei Jin
- Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Ministry of Agriculture and Rural Affairs, Beijing, China
- Wanmei Jin
- Beijing Engineering Research Center of Functional Floriculture, Beijing, China
- DOI
- https://doi.org/10.3389/fpls.2022.857684
- Journal volume & issue
-
Vol. 13
Abstract
Rose (Rosa sp.) flowers have a rich diversity of colors resulting from the differential accumulation of anthocyanins, flavonols, and carotenoids. However, the genetic and molecular determinants of the red-petal trait in roses remains poorly understood. Here we report that a transposable element-like insertion (Rosa1) into RcMYB114, a R2R3-MYB transcription factor’s promoter region causes its transcription, resulting in red petals. In red-petal varieties, RcMYB114 is expressed specifically in flower organs, but is absent from non-red varieties. Sequencing, yeast two-hybrid, transient transformation, and promoter activity assays of RcMYB114 independently confirmed the role of Rosa1 in altering RcMYB114’s transcription and downstream effects on flower color. Genetic and molecular evidence confirmed that the Rosa1 transposable element-like insertion, which is a previously unknown DNA transposable element, is different from those in other plants and is a reliable molecular marker to screen red-petal roses.
Keywords
