Evaluation of the applicability of an automatic method for aflatoxin purification based on immunomagnetic beads

Abstract

The objection was to evaluate the applicability of an automated method for aflatoxins analysis by using Immunoaffinity Magnetic Bead - UPLC. The results showed that the average recovery of the mmunoaffinity Magnetic Bead was above 95%, and the relative standard deviation was less than 3.3%. The results of the quality control samples were within the range of the reference uncertainty. The method was accurate and reliable.T testing (sig. (bilateral) = 0.533) proved that there was no significant difference between two independent purification instruments.62 researchers evaluated two methods of purifications with immunomagnetic beads and immunoaffinity column. T testing has proved that the results of the two methods were no significant difference.However, the dispersion degree of the immunomagnetic bead automated processing method was smaller than that of the immunoaffinity column method. Moreover, the z-value of all the test were less than 2 (| Z | ≤2). Hence, this method can detect cereals and oils. In addition, the method is environmentally friendly, simply, and lower-cost, which greatly improved detection efficiency and level.

Keywords

• immunomagnetic beads
• aflatoxin
• automatic purification
• applicability evaluation
• grain