Five species of black fungus gnats of the genus Bradysia Winnertz, 1867, B. brevispina Tuomikoski, 1960, B. microspina Mohrig & Krivosheina, 1989, B. cinerascens (Grzegorzek, 1884), B. angustoocularis Mohrig & Krivosheina, 1989 and B. polonica (Lengersdorf, 1929) are recorded from Ukraine for the first time. Distributions of these species are summarized and diagnoses of the species are provided.