Cytokine profi le in cows with necrobacterious fingers

Abstract

Orthopedic diseases in cows signifi cantly reduce the profitability of dairy cattle breeding, causing signifi cant technological and economic damage. The aim of the study was to establish a cytokine profi le in cows with necrobacterioses of fi nger lesions. The work was performed in typical dairy farms, on cows with various clinical forms of purulent-necrotic lesions of the extremities of a necrobacterious nature. Diagnostic studies included a complex of clinical, bacteriological biopsy samples of purulent-necrotic foci and determination of the level of cytokines in the blood using enzyme-linked immunosorbent assay. A bacteriological study revealed various associations of F. necrophorum with E. coli, Clostridium spp., Diplococcus lans., Staphylococcus aureus and streptococci, which verifi ed the necrobacterious nature of limb lesions in cows. It was established that in cows with an acute course of purulent-necrotic infl ammatory process in the fi nger area, the concentrations of TNF-α and IL-10 sharply increase. The generalized form of necrobacillary lesions is characterized by a critical increase in the level of TNF-α in the blood by 16.8 times, IL-1β by 17.8 times compared with its level in the acute form of the concentration of IL-10. The cytokine index IL-10: TNF-α acquired a critical value of 0.4: 1, and IL-10 to IL-1β - 1: 1. The development of uncontrolled phlogogenic cytokinemia is noted, which refl ects a systemic infl ammatory response syndrome. A recurrent type of purulent-necrotic lesion of the extremities is characterized by rather low levels of IL-1β and IL-10. The content of IL-1β was 2.4 times greater (p <0.05). However, the concentration of TNF remained quite large and prevailed over the norm by 12.6 times (p <0.001), which was refl ected in the extremely low corresponding index of 0.3: 1. That is, in this case, in a chronic course of necrobacteriosis of the extremities of cows, uncompensated permanent cytokinemia due to TNF-α is formed, which leads to the recurrent nature of the purulent-necrotic process. So, the mechanism of development of the cytokine profi le in cattle requires further research.

Keywords

• phlogogenic and anti-infl ammatory purulent-necrotic lesions
• cytokines
• cows
• infl ammation
• fi ngers