Generation of Macaca fascicularis iPS cell line ATCi-MF1 from adult skin fibroblasts using non-integrative Sendai viruses

Giulia Coppiello; Gloria Abizanda; Natalia Aguado; Elena Iglesias; Estibaliz Arellano-Viera; Juan R Rodriguez-Madoz; Xonia Carvajal-Vergara; Felipe Prosper; Xabier L. Aranguren

doi:10.1016/j.scr.2017.03.008

Stem Cell Research (May 2017)

Generation of Macaca fascicularis iPS cell line ATCi-MF1 from adult skin fibroblasts using non-integrative Sendai viruses

Giulia Coppiello,
Gloria Abizanda,
Natalia Aguado,
Elena Iglesias,
Estibaliz Arellano-Viera,
Juan R Rodriguez-Madoz,
Xonia Carvajal-Vergara,
Felipe Prosper,
Xabier L. Aranguren

Affiliations

Giulia Coppiello: Cell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Health Research Institute of Navarra, Pamplona, Spain
Gloria Abizanda: Cell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Health Research Institute of Navarra, Pamplona, Spain
Natalia Aguado: Cell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Health Research Institute of Navarra, Pamplona, Spain
Elena Iglesias: Cell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Health Research Institute of Navarra, Pamplona, Spain
Estibaliz Arellano-Viera: Cell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Health Research Institute of Navarra, Pamplona, Spain
Juan R Rodriguez-Madoz: Cell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Health Research Institute of Navarra, Pamplona, Spain
Xonia Carvajal-Vergara: Cell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Health Research Institute of Navarra, Pamplona, Spain
Felipe Prosper: Cell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Health Research Institute of Navarra, Pamplona, Spain
Xabier L. Aranguren: Cell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Health Research Institute of Navarra, Pamplona, Spain

DOI: https://doi.org/10.1016/j.scr.2017.03.008
Journal volume & issue: Vol. 21, no. C
pp. 1 – 4

Abstract

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We generated ATCi-MF1 induced pluripotent stem (iPS) cell line from Macaca fascicularis adult skin fibroblasts using non-integrative Sendai viruses carrying OCT3/4, KLF4, SOX2 and c-MYC. Once established, ATCi-MF1 cells present a normal karyotype, are Sendai virus-free and express pluripotency associated markers. Microsatellite markers analysis confirmed the origin of the iPS cells from the parental fibroblasts. Pluripotency was tested with the in vivo teratoma formation assay. ATCi-MF1 cell line may be a useful primate iPS cell model to test different experimental conditions where the use of human cells can imply ethical issues, as microinjection of pluripotent stem cells in pre-implantational embryos.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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