MARCO<sup>+</sup> Macrophage Dynamics in Regenerating Liver after 70% Liver Resection in Mice
Andrey Elchaninov,
Anastasia Lokhonina,
Polina Vishnyakova,
Anna Soboleva,
Anastasiya Poltavets,
Daria Artemova,
Andrey Makarov,
Valeria Glinkina,
Dmitry Goldshtein,
Galina Bolshakova,
Gennady Sukhikh,
Timur Fatkhudinov
Affiliations
Andrey Elchaninov
Laboratory of Regenerative Medicine, National Medical Research Center for Obstetrics, Gynecology and Perinatology Named after Academician V.I. Kulakov of Ministry of Healthcare of Russian Federation, 117997 Moscow, Russia
Anastasia Lokhonina
Laboratory of Regenerative Medicine, National Medical Research Center for Obstetrics, Gynecology and Perinatology Named after Academician V.I. Kulakov of Ministry of Healthcare of Russian Federation, 117997 Moscow, Russia
Polina Vishnyakova
Laboratory of Regenerative Medicine, National Medical Research Center for Obstetrics, Gynecology and Perinatology Named after Academician V.I. Kulakov of Ministry of Healthcare of Russian Federation, 117997 Moscow, Russia
Anna Soboleva
Laboratory of Growth and Development, Scientific Research Institute of Human Morphology, 117418 Moscow, Russia
Anastasiya Poltavets
Laboratory of Regenerative Medicine, National Medical Research Center for Obstetrics, Gynecology and Perinatology Named after Academician V.I. Kulakov of Ministry of Healthcare of Russian Federation, 117997 Moscow, Russia
Daria Artemova
Laboratory of Growth and Development, Scientific Research Institute of Human Morphology, 117418 Moscow, Russia
Andrey Makarov
Histology Department, Medical Institute, Peoples’ Friendship University of Russia (RUDN University), 117198 Moscow, Russia
Valeria Glinkina
Histology Department, Pirogov Russian National Research Medical University, Ministry of Healthcare of the Russian Federation, 117997 Moscow, Russia
Dmitry Goldshtein
Stem Cell Genetics Laboratory, Research Centre for Medical Genetics, 115522 Moscow, Russia
Galina Bolshakova
Laboratory of Growth and Development, Scientific Research Institute of Human Morphology, 117418 Moscow, Russia
Gennady Sukhikh
Laboratory of Regenerative Medicine, National Medical Research Center for Obstetrics, Gynecology and Perinatology Named after Academician V.I. Kulakov of Ministry of Healthcare of Russian Federation, 117997 Moscow, Russia
Timur Fatkhudinov
Histology Department, Medical Institute, Peoples’ Friendship University of Russia (RUDN University), 117198 Moscow, Russia
Background: Macrophages play a key role in liver regeneration. The fates of resident macrophages after 70% resection are poorly investigated. In this work, using the MARCO macrophage marker (abbreviated from macrophage receptor with collagenous structure), we studied the dynamics of mouse liver resident macrophages after 70% resection. Methods: In BALB/c male mice, a model of liver regeneration after 70% resection was reproduced. The dynamics of markers CD68, TIM4, and MARCO were studied immunohistochemically and by using a Western blot. Results: The number of MARCO- and CD68-positive macrophages in the regenerating liver increased 1 day and 3 days after resection, respectively. At the same time, the content of the MARCO protein increased in the sorted macrophages of the regenerating liver on the third day. Conclusions: The data indicate that the number of MARCO-positive macrophages in the regenerating liver increases due to the activation of MARCO synthesis in the liver macrophages. The increased expression of MARCO by macrophages can be regarded as a sign of their activation. In the present study, stimulation with LPS led to an increase in the expression of the Marco gene in both Kupffer cells and macrophages of bone marrow origin.
