Development and characterization of murine monoclonal antibody specific for the P1.4 PorA proteins from strain B:4:P1.(7b).4. of Neisseria meningitidis
María Elena Pérez,
Ramón Barberá,
Francisco Domínguez ,
Oscar Otero,
Mercedes Gutiérrez,
Gustavo Falero,
Grethel Reyes,
Franklin Sotolongo,
Gustavo Sierra
Affiliations
María Elena Pérez
Instituto Finlay. Centro de Investigación y Producción de Vacunas. Ave. 27 No. 19805, La Lisa, A.P. 16017 Cod. 11600. La Habana, Cuba
Ramón Barberá
Instituto Finlay. Centro de Investigación y Producción de Vacunas. Ave. 27 No. 19805, La Lisa, A.P. 16017 Cod. 11600. La Habana, Cuba
Francisco Domínguez
Instituto Finlay. Centro de Investigación y Producción de Vacunas. Ave. 27 No. 19805, La Lisa, A.P. 16017 Cod. 11600. La Habana, Cuba
Oscar Otero
Instituto Finlay. Centro de Investigación y Producción de Vacunas. Ave. 27 No. 19805, La Lisa, A.P. 16017 Cod. 11600. La Habana, Cuba
Mercedes Gutiérrez
Instituto Finlay. Centro de Investigación y Producción de Vacunas. Ave. 27 No. 19805, La Lisa, A.P. 16017 Cod. 11600. La Habana, Cuba
Gustavo Falero
Instituto Finlay. Centro de Investigación y Producción de Vacunas. Ave. 27 No. 19805, La Lisa, A.P. 16017 Cod. 11600. La Habana, Cuba
Grethel Reyes
Instituto Finlay. Centro de Investigación y Producción de Vacunas. Ave. 27 No. 19805, La Lisa, A.P. 16017 Cod. 11600. La Habana, Cuba
Franklin Sotolongo
Instituto Finlay. Centro de Investigación y Producción de Vacunas. Ave. 27 No. 19805, La Lisa, A.P. 16017 Cod. 11600. La Habana, Cuba
Gustavo Sierra
Instituto Finlay. Centro de Investigación y Producción de Vacunas. Ave. 27 No. 19805, La Lisa, A.P. 16017 Cod. 11600. La Habana, Cuba
Neisseria meningitidis isolates are conventionally classified by serosubtyping that characterizes the reactivities of the PorA outer membrane protein variable-region epitopes with monoclonal antibodies. Porins are outer membrane proteins (OMPs) of N. meningitidis serogroup B and have attracted study principally for two reasons: their use in the classification of meningococcal isolates into serotype and subtype and as potential components of vaccines against this important pathogen. New murine hybridomas, secreting specific monoclonal antibodies against PorA serotype P1.4 of N. meningitidis serogroup B, were generated using conventional hybridoma procedures. The monoclonal antibodies obtained were characterized by Western blot and whole cell ELISA, using reference strains from different N. meningitidis serotypes and subtypes. All monoclonal antibodies belong to isotype IgG1. Others hybridomas producing MAbs against PorB and FrpB were also obtained.
