Methodology of Selecting the Optimal Receptor to Create an Electrochemical Immunosensor for Equine Arteritis Virus Protein Detection
Mateusz Brodowski,
Marcin Kowalski,
Wioleta Białobrzeska,
Katarzyna Pałka,
Rafał Walkusz,
Justyna Roguszczak,
Tomasz Łęga,
Marta Sosnowska,
Małgorzata Biedulska,
Joanna Kreczko Kurzawa,
Ewelina Bięga,
Joanna Wysocka,
Marta Lisowska,
Katarzyna Niedźwiedzka,
Tomasz Lipiński,
Sabina Żołędowska,
Dawid Nidzworski
Affiliations
Mateusz Brodowski
Department of Metrology and Optoelectronics, Faculty of Electronics, Telecommunications and Informatics, Gdańsk University of Technology, 11/12 G. Narutowicza St., 80-233 Gdańsk, Poland
Marcin Kowalski
Department of Metrology and Optoelectronics, Faculty of Electronics, Telecommunications and Informatics, Gdańsk University of Technology, 11/12 G. Narutowicza St., 80-233 Gdańsk, Poland
Wioleta Białobrzeska
Institute of Biotechnology and Molecular Medicine, 3 Trzy Lipy St., 80-172 Gdańsk, Poland
Katarzyna Pałka
Institute of Biotechnology and Molecular Medicine, 3 Trzy Lipy St., 80-172 Gdańsk, Poland
Rafał Walkusz
Institute of Biotechnology and Molecular Medicine, 3 Trzy Lipy St., 80-172 Gdańsk, Poland
Justyna Roguszczak
Institute of Biotechnology and Molecular Medicine, 3 Trzy Lipy St., 80-172 Gdańsk, Poland
Tomasz Łęga
Institute of Biotechnology and Molecular Medicine, 3 Trzy Lipy St., 80-172 Gdańsk, Poland
Marta Sosnowska
Institute of Biotechnology and Molecular Medicine, 3 Trzy Lipy St., 80-172 Gdańsk, Poland
Małgorzata Biedulska
Institute of Biotechnology and Molecular Medicine, 3 Trzy Lipy St., 80-172 Gdańsk, Poland
Joanna Kreczko Kurzawa
Institute of Biotechnology and Molecular Medicine, 3 Trzy Lipy St., 80-172 Gdańsk, Poland
Ewelina Bięga
Institute of Biotechnology and Molecular Medicine, 3 Trzy Lipy St., 80-172 Gdańsk, Poland
Joanna Wysocka
Institute of Biotechnology and Molecular Medicine, 3 Trzy Lipy St., 80-172 Gdańsk, Poland
Marta Lisowska
Bioengineering Research Group, Łukasiewicz Research Network–PORT Polish Center for Technology Development, 54-066 Wroclaw, Poland
Katarzyna Niedźwiedzka
Bioengineering Research Group, Łukasiewicz Research Network–PORT Polish Center for Technology Development, 54-066 Wroclaw, Poland
Tomasz Lipiński
Bioengineering Research Group, Łukasiewicz Research Network–PORT Polish Center for Technology Development, 54-066 Wroclaw, Poland
Sabina Żołędowska
Institute of Biotechnology and Molecular Medicine, 3 Trzy Lipy St., 80-172 Gdańsk, Poland
Dawid Nidzworski
Institute of Biotechnology and Molecular Medicine, 3 Trzy Lipy St., 80-172 Gdańsk, Poland
The study reports a methodology of selecting the optimal receptor to create an electrochemical immunosensor for equine arteritis virus (EAV) protein detection. The detection was based on antigen recognition by antibodies immobilized on gold electrodes. Modification steps were controlled by electrochemical impedance spectroscopy and cyclic voltammetry measurements. In order to obtain the impedance immunosensor with the best parameters, seven different receptors complementary to equine arteritis virus protein were used. In order to make the selection, a rapid screening test was carried out to check the sensor’s response to blank, extremely low and high concentrations of target EAV protein, and negative sample: M protein from Streptococcus equi and glycoprotein G from Equid alphaherpesvirus 1. F6 10G receptor showed the best performance.
