Isolation and analyses of lamina propria lymphocytes from mouse intestines

Eunha Kim; Melissa Tran; Yanyi Sun; Jun R. Huh

STAR Protocols (Jun 2022)

Isolation and analyses of lamina propria lymphocytes from mouse intestines

Eunha Kim,
Melissa Tran,
Yanyi Sun,
Jun R. Huh

Affiliations

Eunha Kim: Department of Immunology, Blavatnik Institute, Harvard Medical School, Boston, MA 02115, USA; Corresponding author
Melissa Tran: Department of Immunology, Blavatnik Institute, Harvard Medical School, Boston, MA 02115, USA; Department of Biological Chemistry and Molecular Pharmacology, Blavatnik Institute, Harvard Medical School, Boston, MA 02115, USA
Yanyi Sun: Department of Immunology, Blavatnik Institute, Harvard Medical School, Boston, MA 02115, USA
Jun R. Huh: Department of Immunology, Blavatnik Institute, Harvard Medical School, Boston, MA 02115, USA; Evergrande Center for Immunologic Diseases, Harvard Medical School and Brigham and Women’s Hospital, Boston, MA 02115, USA; Corresponding author

Journal volume & issue: Vol. 3, no. 2
p. 101366

Abstract

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Summary: Investigating intestinal immune responses is critical to understanding local and systemic immunity. However, obtaining resident intestinal immune cells with high cell viability can be challenging. Here, we provide an optimized protocol to isolate lamina propria lymphocytes from the small and large intestines, including lymphocyte activation for cytokine expression analysis and techniques for surface and intracellular antibody staining and flow cytometry. This protocol can be used for isolating and analyzing tissue-resident immune cells from other tissues with specified modifications.For complete details on the use and execution of this protocol, please refer to Kim et al. (2022).

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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