LC/MS guided identification of metabolites of different extracts of Cissus quadrangularis

Abstract

Qualitative and quantitative analysis is very essential to identify the phytochemical constituents present in medicinal plants. In the present study, qualitative and quantitative analyses of a medically important plant, namely Cissus quadrangularis (CQ) were done. Cissus quadrangularis plays a pivotal role in bone healing. We qualitatively analyzed the flavonoids, terpenoids, steroids, starch, saponins, tannins, phenols, glycosides, cardiac glycosides & protein in petroleum ether (PECQ), chloroform (ChCQ), ethanolic (EtCQ) and water (WCQ) extracts of Cissus quadrangularis. Also, UV spectrophotometry of different extracts was carried out to determine the total phenolic content, total alkaloid content and total flavonoid content. Our study demonstrated that phytosterols are present mainly in PECQ and EtCQ extracts. Terpenoids, flavonoids, alkaloids and glycosides are present in all extracts of Cissus quadrangularis.LC/MS data showed that campesterol, ß-sitosterol, stigmasterol, pterostilbene, resveratrol, apigenin-glucoside, genistein, luteolin and prenylated chalcone are present in EtCQ. Coumarin, amino acids, campestanol, astringin, myricetin, quercetin, kaempferol and amyrin are present in PECQ. The ChCQ extract is mainly constituted of phenolic acids, astringin, campesterol, myricetin, quercetin, amyrone and catalpol. The WCQ mainly contains amino acids, phytol, gallic acid, myricetin and catalpol. Pterostilbene and prenylated chalcones were identified for the first time in CQ extracts.

Keywords

• Qualitative analysis
• Quantitative analysis
• Cissus quadrangularis
• Pterostilbene
• Prenylated chalcone and liquid chromatography-mass spectrometry