Potential of Chemically Synthesized Oligosaccharides To Define the Carbohydrate Moieties of the Fungal Cell Wall Responsible for the Human Immune Response, Using <named-content content-type="genus-species">Aspergillus fumigatus</named-content> Galactomannan as a Model
Sarah Sze Wah Wong,
Vadim B. Krylov,
Dmitry A. Argunov,
Alexander A. Karelin,
Jean-Phillipe Bouchara,
Thierry Fontaine,
Jean-Paul Latgé,
Nikolay E. Nifantiev
Affiliations
Sarah Sze Wah Wong
Unité des Aspergillus, Institut Pasteur, Paris, France
Vadim B. Krylov
Laboratory of Glycoconjugate Chemistry, N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
Dmitry A. Argunov
Laboratory of Glycoconjugate Chemistry, N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
Alexander A. Karelin
Laboratory of Glycoconjugate Chemistry, N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
Jean-Phillipe Bouchara
Groupe d’Etude des Interactions Hôte-Pathogène (EA 3142), UNIV Brest, Angers, France
Thierry Fontaine
Unité des Aspergillus, Institut Pasteur, Paris, France
Jean-Paul Latgé
Unité des Aspergillus, Institut Pasteur, Paris, France
Nikolay E. Nifantiev
Laboratory of Glycoconjugate Chemistry, N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
ABSTRACT Methodologies to identify epitopes or ligands of the fungal cell wall polysaccharides influencing the immune response of human pathogens have to date been imperfect. Using the galactomannan (GM) of Aspergillus fumigatus as a model, we have shown that synthetic oligosaccharides of distinct structures representing key fragments of cell wall polysaccharides are the most precise tools to study the serological and immunomodulatory properties of a fungal polysaccharide.
