Molecular understanding of label-free second harmonic imaging of microtubules

V. Van Steenbergen; W. Boesmans; Z. Li; Y. de Coene; K. Vints; P. Baatsen; I. Dewachter; M. Ameloot; K. Clays; P. Vanden Berghe

doi:10.1038/s41467-019-11463-8

Nature Communications (Aug 2019)

Molecular understanding of label-free second harmonic imaging of microtubules

V. Van Steenbergen,
W. Boesmans,
Z. Li,
Y. de Coene,
K. Vints,
P. Baatsen,
I. Dewachter,
M. Ameloot,
K. Clays,
P. Vanden Berghe

Affiliations

V. Van Steenbergen: Laboratory for Enteric NeuroScience (LENS), TARGID, Department of Chronic Diseases Metabolism and Ageing, KU Leuven
W. Boesmans: Laboratory for Enteric NeuroScience (LENS), TARGID, Department of Chronic Diseases Metabolism and Ageing, KU Leuven
Z. Li: Laboratory for Enteric NeuroScience (LENS), TARGID, Department of Chronic Diseases Metabolism and Ageing, KU Leuven
Y. de Coene: Soft matter and Biophysics, Department of Physics and Astronomy, KU Leuven
K. Vints: Electron Microscopy Platform and VIB Bioimaging core facility, VIB-KU Leuven
P. Baatsen: Electron Microscopy Platform and VIB Bioimaging core facility, VIB-KU Leuven
I. Dewachter: Alzheimer Research Group, Biomedical Research Institute, Hasselt University
M. Ameloot: Biophysics, Biomedical Research Institute (BIOMED), Hasselt University
K. Clays: Molecular Imaging and Photonics, Department of Chemistry, KU Leuven
P. Vanden Berghe: Laboratory for Enteric NeuroScience (LENS), TARGID, Department of Chronic Diseases Metabolism and Ageing, KU Leuven

DOI: https://doi.org/10.1038/s41467-019-11463-8
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 14

Abstract

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Microtubules (MTs) are well-studied cytoskeleton components, but have primarily been investigated using fixation or invasive techniques. Here, the authors use label-free second harmonic (SH) fluorescence and correlative light electron microscopy to pinpoint determinants required for SH from MTs.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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