Prediction of Viable CD34 Count in Harvested Product by Peripheral Blood Hematopoietic Progenitor Count of Automated Hematology Analyzer Undergoing Hematopoietic Stem Cell Transplantation

Abstract

Siew Lian Chong,1,* Asral Wirda Ahmad Asnawi,1,2,* Tengku Amatullah Madeehah Tengku Mohd,2,* Sen Mui Tan1,* 1Department of Hematology, Hospital Ampang, Ampang Jaya, Selangor, Malaysia; 2Faculty of Medicine and Health Sciences, Universiti Sains Islam Malaysia, Nilai, Negeri Sembilan, Malaysia*These authors contributed equally to this workCorrespondence: Siew Lian Chong, Department of Hematology, Hospital Ampang, Jalan Mewah Utara, Taman Pandan Mewah, Ampang, Selangor, 68000, Malaysia, Email [email protected] Asral Wirda Ahmad Asnawi, Faculty of Medicine and Health Sciences, Universiti Sains Islam Malaysia, Persiaran Ilmu, Putra Nilai, Nilai, Negeri Sembilan, 71800, Malaysia, Email [email protected]: The CD34+ hematopoietic cell count was used to define cell harvest goals. Successful peripheral blood stem cell transplantation depends on infusion of an appropriate number of HPCs to achieve rapid and durable hematologic recovery.Purpose: In this study, we evaluated the use of the Hematopoietic Progenitor Cell count program on the Sysmex XN-3000 hematology analyzer as an effective parameter for enumerating CD34+ cells.Patients and Methods: Whole blood samples from 144 subjects who are either healthy donors or patients scheduled to undergo peripheral blood stem cell collection were collected and hemopoietic stem cells were quantified using CD34 cell enumeration by flow cytometry and XN-HPC by hematology analyzer.Results: The correlation between the two methods was high (r = 0.766; 95% CI: 0.702– 0.818). Passing–Bablok showed an intercept at 3.45 (2.54 to 4.74) with a slope of 0.78 (95% CI 0.69 to 0.89). Residual analysis of this model indicated no significant deviation from linearity (p = 0.360). The receiver operating characteristic curve demonstrated an area under curve to be 0.88 (0.82 to 0.92), with a positive predictive value of 80.3%. The correlation between CD34+ and XN-HPC showed a strong relationship and good agreement with minimal bias.Conclusion: The XN-HPC showed good analytical performance. With the increasing requirements for stem cell transplantation, a technically simple and rapid alternative for stem cell enumeration that is sustainable is highly useful.Keywords: XN-HPC, CD34+, progenitor, leukemia, allogeneic, autologous

Keywords

• xn-hpc
• cd34+
• progenitor
• leukemia
• allogeneic
• autologous