E3S Web of Conferences (Jan 2024)

Dependence of the reproduction coefficient on donor cells in in vitro improvement of potato cultivars

  • Elmurodov Abdugani,
  • Abdullayeva Yulduz,
  • Obloberdieva Navruza,
  • Kuldashov Bobomurod

DOI
https://doi.org/10.1051/e3sconf/202451001011
Journal volume & issue
Vol. 510
p. 01011

Abstract

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In the experiment, along with growing potato varieties from the meristem cells of the tuber growth, we germinate the botanical seeds of the varieties, grow them from the growth, grow the primary donor cells, obtain healthy callus tissue, grow morphogenetically identical cells, the yield of healthy plant sprouts from the callus tissue is 20% compared to the usual experimental options. 25-30% of callus tissue sprouts, which did not meet the requirements and changed in morphogenesis, remained underdeveloped when callus tissue was grown by taking cells from a normal tumor. It was found that the morphogenesis of the callus sprouts, which were germinated from the seed, obtained from the growths and formed, the weight of the remaining sprouts was 9-10%, and the reproduction coefficient of the sprouts was more than 20%.