Investigating the effect of diluents contained EDTA and Propylene glycol on survival of Ghezel ram frozen semen

Abstract

The present study was performed to evaluate the effect of adding different levels of EDTA and propylene glycol on quality and survival of Ghezel ram frozen semen. After primary evaluations on fresh semen for parameters including total motility, progressive motility, non-progressive motility, viability, abnormal sperm, and acrosome integrity in total cell and acrosome integrity in dead cells, samples with a concentration of 2.5 billion sperm and a progressive motility of over 70% and a volume greater than 0.5 ml were diluted with 1, 1.5, 2, 2.5 mM EDTA and 1, 2, 3% propylene glycol as an additive and propylene glycol 7% as a constitute. Straws (250 µl) were filled with dilution and were placed in a refrigerator for 1.5 to 2 hours to cool to reach 5°C. After cooling they are placed in 4-5 cm above nitrogen for 8-10 minutes and ultimately were immersed in liquid nitrogen. qualitative parameters of sperm included viability, total motility, progressive and non-progressive motility, hypo osmotic swelling test, percentage of acrosome integrity and acrosome health in dead cells were investigated in 0, 20, 40 and 60 days of freezing processes. The results of this experiment showed that diluent with 1% propylene glycol concentration in comparison with other group improved total and progressive motility, percentage of viability, acrosome integrity and HOST (p0.05). The results showed that dilutions of EDTA and propylene glycol could improve quantitative parameters of ram semen.

Keywords

• edta
• freezing
• ghezel ram
• propylene glycol
• sperm cryopreservation