Stem Cell Research (Mar 2016)

Generation of iPSC line iPSC-FH2.1 in hypoxic conditions from human foreskin fibroblasts

  • María Questa,
  • Leonardo Romorini,
  • Carolina Blüguermann,
  • Claudia María Solari,
  • Gabriel Neiman,
  • Carlos Luzzani,
  • María Élida Scassa,
  • Gustavo Emilio Sevlever,
  • Alejandra Sonia Guberman,
  • Santiago Gabriel Miriuka

DOI
https://doi.org/10.1016/j.scr.2015.12.026
Journal volume & issue
Vol. 16, no. 2
pp. 300 – 303

Abstract

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Human foreskin fibroblasts were used to generate the iPSC line iPSC-FH2.1 using the EF1a-hSTEMCCA-loxP vector expressing OCT4, SOX2, c-MYC and KLF4, in 5% O2 culture conditions. Stemness was confirmed, as was pluripotency both in vivo and in vitro, in normoxia and hypoxia. Human Embryonic Stem Cell (hESC) line WA-09 and reprogrammed fibroblast primary culture HFF-FM were used as controls.