International Journal of Nanomedicine (Aug 2023)
Highly Water-Dispersed and Stable Deinoxanthin Nanocapsule for Effective Antioxidant and Anti-Inflammatory Activity
Abstract
Sohyeon Yu,1,2 Sangwoo Kim,1,3 Jisu Kim,1,3 Ji-Woong Kim,4 Su Young Kim,4 Bongjun Yeom,2 Hyungjun Kim,5 Won II Choi,1 Daekyung Sung1 1Center for Bio-Healthcare Materials, Bio-Convergence Materials R&D Division, Korea Institute of Ceramic Engineering and Technology, Cheongju, 28160, Republic of Korea; 2Department of Chemical Engineering, Hanyang University, Seoul, 04763, Republic of Korea; 3Department of Chemical and Biomolecular Engineering, Yonsei University, Seoul, 03722, Republic of Korea; 4Materials Science Research Institute, LABIO Co., Ltd, Seoul, 08501, Republic of Korea; 5Department of Chemistry and Bioscience, Kumoh National Institute of Technology, Gyeongbuk, 39177, Republic of KoreaCorrespondence: Won II Choi; Daekyung Sung, Center for Bio-Healthcare Materials, Bio-Convergence Materials R&D Division, Korea Institute of Ceramic Engineering and Technology, Cheongju, 28160, Republic of Korea, Tel +82-43-913-1513 ; +82-43-913-1511, Fax +82-43-913-1597, Email [email protected]; [email protected]: Deinoxanthin (DX), a carotenoid, has excellent antioxidant and anti-inflammatory properties. However, owing to its lipophilicity, it is unfavorably dispersed in water and has low stability, limiting its application in cosmetics, food, and pharmaceuticals. Therefore, it is necessary to study nanoparticles to increase the loading capacity and stability of DX.Methods: In this study, DX-loaded nanocapsules (DX@NCs) were prepared by nanoprecipitation by loading DX into nanocapsules. The size, polydispersity index, surface charge, and morphology of DX@NCs were confirmed through dynamic light scattering and transmission electron microscopy. The loading content and loading efficiency of DX in DX@NCs were analyzed using high-performance liquid chromatography. The antioxidant activity of DX@NCs was evaluated by DPPH assay and in vitro ROS. The biocompatibility of DX@NCs was evaluated using an in vitro MTT assay. In vitro NO analysis was performed to determine the effective anti-inflammatory efficacy of [email protected]: DX@NCs exhibited increased stability and antioxidant efficacy owing to the improved water solubility of DX. The in situ and in vitro antioxidant activity of DX@NCs was higher than that of unloaded DX. In addition, it showed a strong anti-inflammatory effect by regulating the NO level in an in vitro cell model.Conclusion: This study presents a nanocarrier to improve the water-soluble dispersion and stability of DX. These results demonstrate that DX@NC is a carrier with excellent stability and has a high potential for use in cosmetic and pharmaceutical applications owing to its antioxidant and anti-inflammatory effects.Keywords: deinoxanthin, nanoprecipitation, reactive oxygen species scavenging, antioxidant activity, anti-inflammation
