Efficient labeling and imaging of protein-coding genes in living cells using CRISPR-Tag

Baohui Chen; Wei Zou; Haiyue Xu; Ying Liang; Bo Huang

doi:10.1038/s41467-018-07498-y

Nature Communications (Nov 2018)

Efficient labeling and imaging of protein-coding genes in living cells using CRISPR-Tag

Baohui Chen,
Wei Zou,
Haiyue Xu,
Ying Liang,
Bo Huang

Affiliations

Baohui Chen: Department of Cell Biology, and Bone Marrow Transplantation Center of the First Affiliated Hospital, Zhejiang University School of Medicine
Wei Zou: The Fourth Affiliated Hospital, Zhejiang University School of Medicine
Haiyue Xu: Department of Cell Biology, and Bone Marrow Transplantation Center of the First Affiliated Hospital, Zhejiang University School of Medicine
Ying Liang: Department of Cell Biology, and Bone Marrow Transplantation Center of the First Affiliated Hospital, Zhejiang University School of Medicine
Bo Huang: Department of Pharmaceutical Chemistry, University of California, San Francisco

DOI: https://doi.org/10.1038/s41467-018-07498-y
Journal volume & issue: Vol. 9, no. 1
pp. 1 – 9

Abstract

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Difficulties in imaging non-repetitive genes impair our ability to explore their dynamics. Here the authors present CRISPR-Tag, which marks genes of interest with small repeat sequences to recruit dCas9-GFP.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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