Establisment of Methodology for Identification of Insect-Parasitic Fungi by Molecular Analysis on ITS1-5.8S-ITS2 Region

Abstract

Insect-parastitic fungi, especially Cordyceps sinensis, parasitize larvae of insects, are used as traditional medicinal drugs in various Asian countries, including Vietnam. However, knowledge of taxonomy of these fungi is still limited, due to many factors such as the difficulties in the classification and identification. Recently, the identification of these fungi was improved by molecular and bioinformatic methods. In this preliminarystudy, first we want to establishthe protocol including DNA extraction, PCR, sequencing then proof-reading and phylogenetic tree construction. The target sequence used for this protocol is Internal transcribed spacer –ITS. The protocol is carried out using the sample set, in which the already identified results by morphology and molecular biology combined bioinformatics were in high corcordance. Therefore, this protocol will be applied in order to identifyinsect-parasitic fungi specimens collected from Langbian mountain, Dalat, Lamdong, Vietnam.

Keywords

• cordyceps
• ctab
• hiệu chỉnh trình tự
• its1-5.8s-its2
• phả hệ phân tử