Comparison and Optimization of Saccharification Conditions of Alkaline Pre-Treated Triticale Straw for Acid and Enzymatic Hydrolysis Followed by Ethanol Fermentation
Rafał Łukajtis,
Karolina Kucharska,
Iwona Hołowacz,
Piotr Rybarczyk,
Katarzyna Wychodnik,
Edyta Słupek,
Paulina Nowak,
Marian Kamiński
Affiliations
Rafał Łukajtis
Faculty of Chemistry, Department of Chemical and Process Engineering, Gdańsk University of Technology, Narutowicza 11/12 Street, 80-233 Gdańsk, Poland
Karolina Kucharska
Faculty of Chemistry, Department of Chemical and Process Engineering, Gdańsk University of Technology, Narutowicza 11/12 Street, 80-233 Gdańsk, Poland
Iwona Hołowacz
Faculty of Chemistry, Department of Chemical and Process Engineering, Gdańsk University of Technology, Narutowicza 11/12 Street, 80-233 Gdańsk, Poland
Piotr Rybarczyk
Faculty of Chemistry, Department of Chemical and Process Engineering, Gdańsk University of Technology, Narutowicza 11/12 Street, 80-233 Gdańsk, Poland
Katarzyna Wychodnik
Faculty of Chemistry, Department of Chemical and Process Engineering, Gdańsk University of Technology, Narutowicza 11/12 Street, 80-233 Gdańsk, Poland
Edyta Słupek
Faculty of Chemistry, Department of Chemical and Process Engineering, Gdańsk University of Technology, Narutowicza 11/12 Street, 80-233 Gdańsk, Poland
Paulina Nowak
Faculty of Chemistry, Department of Chemical and Process Engineering, Gdańsk University of Technology, Narutowicza 11/12 Street, 80-233 Gdańsk, Poland
Marian Kamiński
Faculty of Chemistry, Department of Chemical and Process Engineering, Gdańsk University of Technology, Narutowicza 11/12 Street, 80-233 Gdańsk, Poland
This paper concerns the comparison of the efficiency of two-stage hydrolysis processes, i.e., alkaline pre-treatment and acid hydrolysis, as well as alkaline pre-treatment followed by enzymatic hydrolysis, carried out in order to obtain reducing sugars from triticale straw. For each of the analyzed systems, the optimization of the processing conditions was carried out with respect to the glucose yield. For the alkaline pre-treatment, an optimal catalyst concentration was selected for constant values of temperature and pre-treatment time. For enzymatic hydrolysis, optimal process time and concentration of the enzyme preparation were determined. For the acidic hydrolysis, performed with 85% phosphoric acid, the optimum temperature and hydrolysis time were determined. In the hydrolysates obtained after the two-stage treatment, the concentration of reducing sugars was determined using HPLC. The obtained hydrolysates were subjected to ethanol fermentation. The concentrations of fermentation inhibitors are given and their effects on the alcoholic fermentation efficiency are discussed.
