The use of a novel amplification tool for molecular diagnosis of challenging samples

Abstract

Objective: To obtain adequate amounts of starting material to beused in molecular diagnosis. Methods: Isothermal ramificationamplification. Results: Water samples where Legionella spp wasdiluted and small amount of human DNA from HIV infected patientswere submitted to a new amplification technology (GenomiPhi®kit). Those samples that were previously negative upon PCRbecame positive after the treatment. Conclusion: The newtechnology allows for molecular assays to be performed in samplesof reduced volumes or low concentration.

Keywords

• Legionella/isolation & purification
• Legionella/growth & development
• Molecular diagnostic techniques/methods
• HIV