Stem Cell Research (Oct 2021)

An INSULIN-GFP/GLUCAGON-mCherry reporter line for the study of human pancreatic endocrine cell development

  • Tanya Labonne,
  • Andrew G. Elefanty,
  • Edouard G. Stanley,
  • Jacqueline V. Schiesser

Journal volume & issue
Vol. 56
p. 102547


Read online

Insulin expressing beta cells and glucagon expressing alpha cells are the two most abundant endocrine cell types of the human pancreatic islet. Both alpha and beta cells can be generated in vitro via the differentiation of pluripotent stem cells (PSCs), affording the opportunity to study their ontogeny and to examine their developmental inter-relationship. To aid these analyses, we have generated a PSC line in which insulin expression is reported by GFP and glucagon expression is reported by mCherry. This cell line enables viable isolation of cells expressing each hormone and optimisation of methods that lead to their generation.