Journal of Lipid Research (Jul 1979)

Isolation and characterization of a heptaglycosylceramide from bovine erythrocyte membranes.

  • J L Chien,
  • S C Li,
  • Y T Li

Journal volume & issue
Vol. 20, no. 5
pp. 669 – 673

Abstract

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A heptaglycosylceramide was isolated from bovine erythrocyte membranes. The structure was characterized to be Gal(alpha 1-3)Gal(beta 1-4)GlcNAc(beta1-3)Gal(beta 1-4)Glc-NAc(beta 1-4)al(beta 1-4)GlcCer. A hexaglycosylceramide that has the same sequence except for the terminal alpha-galactosyl unit has also been isolated. We have previously found that gangliosides isolated from bovine erythrocyte membranes contain a keratan sulfate type repeating unit –[3Gal(beta 1-4)-GlcNAc beta]–n. This study shows that the keratan sulfate type repeating unit is also present in the neutral glycosphingolipids of bovine erythrocyte membranes.