Mathematical modeling and simulation of newly isolated bacillus cereus M1GT for tannase production through semi-solid state fermentation with agriculture residue triphala

Abstract

ABSTRACT: In this present research, tannase producing bacteria were isolated from the samples of the gastrointestinal tract of a Goat. The liquid enrichment and spread plate technique were adopted and 6 distinct bacterial colonies were isolated on a nutrient agar plate. Based on qualitative and quantitative methods of screening, one isolate among six was found promising and identified as Bacillus cereus M1GT by using 16S rRNA gene sequencing technique. The cost-effective substrate Triphala was used as a tannin and energy source for tannase production through solid-state fermentation in shake flask independently. Process factors were screened by a two-step optimization process i.e. one factor at a time method and central composite design. The study of statistical experimental design with Triphala at optimized conditions showed 6.1-fold (0.116 U/gds) higher in tannase activity than that obtained in submerged fermentation. Further, the kinetics of Bacillus cereus M1GT under optimum process conditions were studied. The Logistic equation (growth, ‘µ’), Luedeking Piret equation (Tannase, ‘α’, and ‘β’) and Substrate utilization equation (Tannic acid, ‘m’ and ‘n’) of unstructured kinetic models were evaluated with the MATLAB program. The experimental and simulated values exhibited a good correspondence, indicating that models will define tannase production process.

Keywords

• Tannase
• Bacillus cereus M1GT
• Triphala
• Process optimization
• Semi-solid state fermentation
• Unstructured kinetic model